Tamatani S, Ozawa T, Minakawa T, Takeuchi S, Koike T, Tanaka R
Department of Neurosurgery, Niigata University, Japan.
J Neurosurg. 1997 Jan;86(1):109-12. doi: 10.3171/jns.1997.86.1.0109.
This study was undertaken to evaluate the histological reaction of cultured endothelial cells to endovascular embolic materials in vitro. Endothelial cells were isolated and cultured from a canine carotid artery. Embolic materials (platinum microcoils, polyvinyl alcohol particles, silicon balloons, or silk threads), either in their normal state or after having been coated with type 1 collagen, fibronectin, or laminin, were placed on endothelial cells and cocultured for 6, 12, and 24 hours and 2, 3, 7, 14, and 21 days. The cocultures were investigated histologically using a scanning electron microscope. Endothelial cells were not found on any uncoated embolic materials, even at 21 days. On the materials coated with fibronectin or laminin, endothelial cells began to proliferate in 7 days, covering the materials extensively in 14 days. On the other hand, endothelial cells began to proliferate on the collagen-coated materials in 3 days, covering them extensively in 7 days and reaching confluence with a cobblestone pattern in 21 days. The densities of endothelial cells on collagen-coated materials were much higher than those observed on the materials coated with other extracellular matrices. Future advantages of the clinical use of collagen-coated embolic materials in interventional treatment are discussed.
本研究旨在评估体外培养的内皮细胞对血管内栓塞材料的组织学反应。从犬颈动脉分离并培养内皮细胞。将处于正常状态或已用I型胶原、纤连蛋白或层粘连蛋白包被的栓塞材料（铂微线圈、聚乙烯醇颗粒、硅胶球囊或丝线）置于内皮细胞上，共培养6、12和24小时以及2、3、7、14和21天。使用扫描电子显微镜对共培养物进行组织学研究。即使在21天时，在任何未包被的栓塞材料上均未发现内皮细胞。在纤连蛋白或层粘连蛋白包被的材料上，内皮细胞在7天时开始增殖，14天时广泛覆盖材料。另一方面，内皮细胞在胶原包被的材料上3天时开始增殖，7天时广泛覆盖，21天时达到鹅卵石样汇合。胶原包被材料上内皮细胞的密度远高于其他细胞外基质包被材料上观察到的密度。讨论了胶原包被栓塞材料在介入治疗中临床应用的潜在优势。
