Unseld M, Marienfeld J R, Brandt P, Brennicke A
Institut für Genbiologische Forschung, Berlin, Germany.
Nat Genet. 1997 Jan;15(1):57-61. doi: 10.1038/ng0197-57.
We have determined the complete sequence of the mitochondrial DNA in the model plant species Arabidopsis thaliana, affording access to the first of its three genomes. The 366,924 nucleotides code for 57 identified genes, which cover only 10% of the genome. Introns in these genes add about 8%, open reading frames larger than 100 amino acids represent 10% of the genome, duplications account for 7%, remnants of retrotransposons of nuclear origin contribute 4% and integrated plastid sequences amount to 1%-leaving 60% of the genome unaccounted for. With the significant contribution of duplications, imported foreign DNA and the extensive background of apparently functionless sequences, the mosaic structure of the Arabidopsis thaliana mitochondrial genome features many aspects of size-relaxed nuclear genomes.
我们已经确定了模式植物拟南芥线粒体DNA的完整序列，从而能够获取其三个基因组中的首个基因组序列。这366,924个核苷酸编码了57个已鉴定的基因，这些基因仅覆盖基因组的10%。这些基因中的内含子约占8%，大于100个氨基酸的开放阅读框占基因组的10%，重复序列占7%，核起源反转录转座子的残余部分占4%，整合的质体序列占1%，其余60%的基因组未得到解释。由于重复序列、导入的外源DNA以及大量明显无功能序列的显著贡献，拟南芥线粒体基因组的镶嵌结构具有许多大小宽松的核基因组的特征。
