Garretta M, Benamon D, Drouet J, Courocé-Pauty A M, Gener J, Muller A, Soulier J P
Vox Sang. 1977 Sep;33(3):175-86. doi: 10.1111/j.1423-0410.1977.tb02250.x.
The widespread utilization of Groupamatic equipment for routine immunohaematological tests has increased the demand for serological procedures that can be performed with this machine. We describe the test method which was developed and with which the detection of HBs antigen has now been carried out routinely for 1 year. It is an automated haemagglutination inhibition reaction which is called GIPHA (Groupamatic inhibition of passive haemagglutination). The sensitivity corresponds to 2 CNTS units, the antigen weight for 1 unit being from 1 to 4 ng. The reproducibility is good; the percentage of false positive reactions is about 0.5% and that of technical problems 0.7%. The additional advantages of the machines are numerous, among which we have the possibility of 11 other simultaneous reactions. One of them is the detection of antibodies against HBs antigen, which is a passive haemagglutination test called PHAG (passive haemagglutination on Groupamatic).
Groupamatic设备在常规免疫血液学检测中的广泛应用，增加了对可使用该机器进行的血清学检测程序的需求。我们描述了所开发的检测方法，利用该方法检测乙肝表面抗原现已常规进行了1年。这是一种自动血凝抑制反应，称为GIPHA（Groupamatic被动血凝抑制）。灵敏度相当于2个CNTS单位，1个单位的抗原重量为1至4纳克。重复性良好；假阳性反应的百分比约为0.5%，技术问题的百分比为0.7%。该机器还有许多其他优点，其中包括可以同时进行11种其他反应。其中之一是检测抗乙肝表面抗原抗体，这是一种称为PHAG（Groupamatic上的被动血凝）的被动血凝试验。
