Hou X E, Dahlström A
Department of Anatomy and Cell Biology, Göteborg University, Sweden.
J Auton Nerv Syst. 1996 Dec 14;61(3):301-12. doi: 10.1016/s0165-1838(96)00100-2.
The cells of sympathoadrenal lineage display different characteristics after differentiation, although they stem from the same neural crest precursor during embryonic development. In the present study we compared the distribution patterns of several synaptic vesicle proteins in the superior cervical ganglion (SCG) and the adrenal medulla. Using indirect immunofluorescence combined with confocal laser scanning microscopy, it was observed that antisera against integral synaptic vesicle membrane proteins (SV2, synaptotagmin I, synaptobrevin II and synaptophysin) induced strong immunoreactivities in these cells, but anti-synaptobrevin I caused only a faint fluorescence. Immunoreactivities of the synaptic vesicle-associated proteins Rab3a and SNAP25 were also observed in the cells. Synapsin-Ia-reactive material appeared absent from chromaffin cells but present in small amounts in sympathetic neurons in the SCG and iris terminals. On the other hand, synapsin IIa immunoreactive material was strong in most SCG neurons and in adrenergic iris terminals. The neural specific clatrin light chain was detected in the SCG cells and in ganglion cells of the adrenal, but only weak traces could be observed in chromaffin cells. One of the vesicular monoamine transmitter transporters, VMAT2, which is expressed in catecholamine neurons in the brain stem, was observed in most cells in the SCG and also in groups of cells in the adrenal medulla, where the VMAT2-positive small chromaffin cells were PNMT-negative. SIF cells in the SCG contained most of the synaptic vesicle proteins investigated. The results show that after differentiation, sympathetic neurons, SIF cells and adrenal chromaffin cells still share many vesicle proteins even though their physiology is different.
交感肾上腺谱系的细胞在分化后表现出不同的特征，尽管它们在胚胎发育过程中起源于相同的神经嵴前体。在本研究中，我们比较了几种突触囊泡蛋白在上颈神经节（SCG）和肾上腺髓质中的分布模式。使用间接免疫荧光结合共聚焦激光扫描显微镜观察到，针对完整突触囊泡膜蛋白（SV2、突触结合蛋白I、突触小泡蛋白II和突触素）的抗血清在这些细胞中诱导出强烈的免疫反应，但抗突触小泡蛋白I仅引起微弱的荧光。在这些细胞中也观察到了突触囊泡相关蛋白Rab3a和SNAP25的免疫反应。嗜铬细胞中似乎不存在突触素-Ia反应性物质，但在SCG和虹膜终末的交感神经元中少量存在。另一方面，突触素IIa免疫反应性物质在大多数SCG神经元和肾上腺素能虹膜终末中很强。在SCG细胞和肾上腺神经节细胞中检测到了神经特异性网格蛋白轻链，但在嗜铬细胞中仅观察到微弱的痕迹。囊泡单胺转运体之一VMAT2在脑干的儿茶酚胺能神经元中表达，在SCG的大多数细胞以及肾上腺髓质的细胞群中也观察到了VMAT2，其中VMAT2阳性的小嗜铬细胞是PNMT阴性的。SCG中的SIF细胞含有大多数所研究的突触囊泡蛋白。结果表明，分化后，交感神经元、SIF细胞和肾上腺嗜铬细胞尽管生理功能不同，但仍共享许多囊泡蛋白。
