半胱胺对溴脱氧尿苷（BUdR）取代的人类染色体鉴别染色的影响。

Influence of cysteamine on differential staining of BUdR-substituted human chromosomes.

作者信息

Scheid W

出版信息

Can J Genet Cytol. 1979 Mar;21(1):145-9. doi: 10.1139/g79-019.

DOI:10.1139/g79-019

PMID:89890

Abstract

In 5-bromodeoxyuridine (BUdR)-substituted human chromsomes stained with 4'-6-diamidino-2-phenylindole (DAPI) differential staining is suppressed totally by the H+-donor cysteamine (concentration 0.08 M). We propose that differential staining appears because the double BUdR-substituted chromatid will be disintegrated via a photosensitive dye-visible light system. It is suggested that cysteamine prevents the production of strand breaks in DNA and, consequently, differential staining in BUdR-substituted chromosomes. Furthermore it is shown that differential staining with DAPI causes irreversible changes in the double BUdR-substituted chromatid. This finding can be explained with the above mentioned mechanism.

摘要

在用4′-6-二脒基-2-苯基吲哚（DAPI）染色的5-溴脱氧尿苷（BUdR）取代的人类染色体中，H⁺供体半胱胺（浓度0.08 M）完全抑制了差异染色。我们提出差异染色的出现是因为双BUdR取代的染色单体将通过光敏染料-可见光系统解体。有人认为半胱胺可防止DNA链断裂的产生，从而防止BUdR取代的染色体出现差异染色。此外，还表明用DAPI进行的差异染色会导致双BUdR取代的染色单体发生不可逆变化。这一发现可用上述机制来解释。

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Can J Genet Cytol. 1979 Mar;21(1):145-9. doi: 10.1139/g79-019.

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引用本文的文献

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DNA lysis is involved in the simplified fluorescence plus Giemsa method for differential staining of sister chromatids.DNA裂解参与了姐妹染色单体差异染色的简化荧光加吉姆萨法。

Chromosoma. 1984;89(1):76-8. doi: 10.1007/BF00302354.

2

Different effects of 33258 Hoechst and DAPI in fluorescent staining of sister chromatids differentially substituted with bromodeoxyuridine.33258 Hoechst和DAPI在对用溴脱氧尿苷进行不同取代的姐妹染色单体荧光染色中的不同作用。

Histochemistry. 1982;75(2):169-77. doi: 10.1007/BF00496008.

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