Galvan L, Strong J E, Crooke S T
Cancer Res. 1979 Oct;39(10):3948-51.
The PM-2 DNA fluorescence assay has been shown to be a rapid, sensitive, and reproducible assay for bleomycin biochemical activity. The assay can detect bleomycin in human serum in the nmol range. The method measures DNA degradative activity of bleomycin and could be used to determine activity of bleomycin analogs and metabolites. The usefulness of the assay to perform bleomycin pharmacokinetic studies in cancer patients has been demonstrated. Linear regression analyses of parallel bleomycin assays with the radioimmunoassay gave a coefficient of correlation of 0.98 to 0.78 with trichloroacetic acid-treated serum. These results indicate excellent agreement between the two assays.
PM - 2 DNA荧光测定法已被证明是一种用于博来霉素生化活性的快速、灵敏且可重复的测定方法。该测定法能够检测人血清中纳摩尔范围内的博来霉素。此方法可测量博来霉素的DNA降解活性，还可用于确定博来霉素类似物和代谢产物的活性。已证明该测定法在癌症患者中进行博来霉素药代动力学研究的实用性。用放射免疫测定法对平行的博来霉素测定进行线性回归分析，对于经三氯乙酸处理的血清，相关系数为0.98至0.78。这些结果表明两种测定法之间具有极好的一致性。
