Elson M K, Oken M M, Shafer R B
J Nucl Med. 1977 Mar;18(3):296-9.
A radioimmunoassay for bleomycin, suitable for monitoring serum and urine drug levels, has been developed. Antisera were obtained from rabbits immunized with a conjugate of bleomycin and human serum albumin.Bleomycin labeled with 57Co was used as the tracer. Antibody-bound and free bleomycin were separated by precipitation with polyethylene glycol. The assay could determine bleomycin levels down to 0.025 mug/ml. The assay was precise, with a coefficient of variation of 2.8% Other drugs likely to be administered in combination with bleomycin did not interfere with the radioimmunoassay.
已开发出一种适用于监测血清和尿液中博来霉素药物水平的放射免疫分析法。抗血清取自用博来霉素与人血清白蛋白的结合物免疫的兔子。用57Co标记的博来霉素作为示踪剂。通过用聚乙二醇沉淀来分离抗体结合的和游离的博来霉素。该分析法可测定低至0.025微克/毫升的博来霉素水平。该分析法精确，变异系数为2.8%。其他可能与博来霉素联合使用的药物不会干扰放射免疫分析法。
