Dow B C, Buchanan I, Munro H, Follett E A, Davidson F, Prescott L E, Yap P L, Simmonds P
Regional Virus Laboratory, Ruchill Hospital, Glasgow.
J Med Virol. 1996 Jun;49(2):132-6. doi: 10.1002/(SICI)1096-9071(199606)49:2<132::AID-JMV10>3.0.CO;2-G.
HCV antibody screening of 624,910 blood donations resulted in 3,832 samples being referred for confirmation. All were tested by RIBA-3 with 2,710 negative, 945 indeterminate and 177 positive results. HCV RNA was detected by PCR in an average of 69.5% of RIBA-3 positives (4 bands 84.1%; 3 bands 74.1%; 2 bands 34.1%) and only 0.53% of RIBA-3 indeterminates. Eighty-four percent of samples with a total RIBA-3 band intensity score (maximum 16) of > or = 8 were PCR positive compared with only 22% of those with a score of < 8. Total mean band intensities for HCV genotype 1 samples (n = 65) were 13.2, genotype 2 (n = 17) 11.4 and genotype 3 (n = 65) 11.2 with type 1 samples showing greater reactivity with c100 and c33 antibodies. No PCR positive type 1 samples were found with RIBA-3 total band scores less than 8, no PCR positive type 2 samples less than 6, whilst PCR positive type 3 samples were found with scores as low as 2. NS5 indeterminates were the most common (40.2%) single band pattern but yielded no PCR positive samples, followed by c33 (23.3%) with one PCR positive and c100 (20.2%) with one PCR positive whilst c22 indeterminates were least common (16.3%) but included three PCR positive donors. All five RIBA-3 indeterminate PCR positive donors were type 3.
对624,910份献血样本进行丙肝病毒（HCV）抗体筛查，结果有3,832份样本被送去做确认检测。所有样本均采用重组免疫印迹分析-3（RIBA-3）检测，其中2,710份结果为阴性，945份结果不确定，177份结果为阳性。通过聚合酶链反应（PCR）检测发现，平均69.5%的RIBA-3阳性样本（4条带的样本占84.1%；3条带的样本占74.1%；2条带的样本占34.1%）以及仅0.53%的RIBA-3结果不确定样本检测出HCV核糖核酸（RNA）。RIBA-3总条带强度评分（最高16分）≥8分的样本中，84%的样本PCR检测呈阳性，而评分<8分的样本中该比例仅为22%。HCV 1型样本（n = 65）的总平均条带强度为13.2，2型样本（n = 17）为11.4，3型样本（n = 65）为11.2，1型样本与c100和c33抗体的反应性更强。RIBA-3总条带评分低于8分的样本中未发现PCR阳性的1型样本，低于6分的样本中未发现PCR阳性的2型样本，而PCR阳性的3型样本评分低至2分。非结构蛋白5（NS5）结果不确定是最常见的（40.2%）单一条带模式，但未产生PCR阳性样本，其次是c33（23.3%），有1份PCR阳性样本，c100（20.2%）有1份PCR阳性样本，而c22结果不确定的情况最不常见（16.3%），但包括3份PCR阳性献血者样本。所有5份RIBA-3结果不确定且PCR阳性的献血者样本均为3型。
