Behzad A R, Chu F, Walker D C
Pulmonary Research Laboratory, St. Paul's Hospital, University of British Columbia, Vancouver, Canada.
Microvasc Res. 1996 May;51(3):303-16. doi: 10.1006/mvre.1996.0029.
Previous findings have shown that pulmonary fibroblasts are associated with preexisting holes in the endothelial and epithelial basal laminae through which neutrophils appear to enter and leave the interstitium as they migrate from capillaries to alveoli. To determine their role in neutrophil migration, fibroblast organization within the interstitium was assessed by transmission electron microscope observations of serial-sectioned rabbit lung tissue. Interstitial fibroblasts were found to physically interconnect the endothelial basal lamina holes to epithelial basal lamina holes. Morphometric assessment of rabbit lung tissue instilled with Streptococcus pneumoniae revealed that approximately 70% of the surface area density of migrating neutrophils is in close contact (15 nm or less) with interstitial fibroblasts and extracellular matrix elements (30 and 40%, respectively). Although migrating neutrophils were close enough to adhere to both fibroblasts and extracellular elements, the interstitial fibroblasts are organized in a manner that would allow them to provide directional information to the neutrophils. A model illustrating this process is proposed.
先前的研究结果表明，肺成纤维细胞与内皮和上皮基底层中预先存在的孔洞相关，当嗜中性粒细胞从毛细血管迁移到肺泡时，它们似乎通过这些孔洞进出间质。为了确定它们在嗜中性粒细胞迁移中的作用，通过对连续切片的兔肺组织进行透射电子显微镜观察，评估了间质内成纤维细胞的组织情况。发现间质成纤维细胞在物理上把内皮基底层孔洞与上皮基底层孔洞连接起来。对注入肺炎链球菌的兔肺组织进行形态计量学评估显示，迁移的嗜中性粒细胞表面积密度约70%与间质成纤维细胞和细胞外基质成分紧密接触（分别为15纳米或更小）（分别占30%和40%）。虽然迁移的嗜中性粒细胞距离足够近，可以与成纤维细胞和细胞外成分都发生黏附，但间质成纤维细胞的组织方式使其能够为嗜中性粒细胞提供方向信息。本文提出了一个说明这一过程的模型。
