Arnaudov A, Tziporkov N
Regional Veterinary Research Institute, Plovdiv, Bulgaria.
Biotherapy. 1996;9(1-3):105-8. doi: 10.1007/BF02628666.
From a rabbit lymphoid tissue, twice immunized with a Salmonella ch. suis vaccine, was obtained a dialysable leucocyte extract (DLE) (m. w. 10,000Da; protein content 1.14 mg/ml; content of ribose 2.7 mg/ml; A260/A280 ratio 2.17 and pH 6.8). By gel filtration on Sephadex G-25, six peaks were obtained and activity was found in peak IV. The activity of the extract was determined by a dermo-application test (DAT) on 10 cows. The protective effect was tested by a challenge with Salmonella ch. suis and Salmonella dublin pathogen strains on white mice intraperitoneally treated with DLE. The DAT proved to be positive in 8 of the 10 cows. When applied on white mice, it induced a high specific protective effect against Salmonella ch. suis (70%), but not against Salmonella dublin infection.
从用猪霍乱沙门氏菌疫苗进行过两次免疫的兔淋巴组织中，获得了一种可透析白细胞提取物（DLE）（分子量10,000道尔顿；蛋白质含量1.14毫克/毫升；核糖含量2.7毫克/毫升；A260/A280比值2.17，pH值6.8）。通过在葡聚糖G - 25上进行凝胶过滤，得到了六个峰，且在峰IV中发现了活性。提取物的活性通过对10头奶牛进行皮肤应用试验（DAT）来测定。通过用猪霍乱沙门氏菌和都柏林沙门氏菌病原体菌株对经DLE腹腔注射处理的小白鼠进行攻毒试验来测试其保护效果。DAT在10头奶牛中的8头中被证明为阳性。当应用于小白鼠时，它对猪霍乱沙门氏菌诱导了高度的特异性保护作用（70%），但对都柏林沙门氏菌感染没有保护作用。
