Mikula I, Pistl J, Rosocha J
Department of Microbiology, Immunology and Animal Hygiene, University of Veterinary Medicine, Kosice, Czechoslovakia.
Vet Immunol Immunopathol. 1992 Apr;32(1-2):103-21. doi: 10.1016/0165-2427(92)90072-x.
Activity of Salmonella-specific dialyzable leukocyte extracts (DLE) prepared from mesenteric lymphatic nodes of calves and stabilized with bovine albumin was studied in this work. The effect of ambient temperature and storage period on the activity of DLE was evaluated. Testing for DLE activity by means of capillary leukocyte migration inhibition (LMI) assay showed that DLE stabilized with albumin retained 60% of its activity for 12 months of storage at 4 degrees C. This level of activity was retained in the native DLE (without albumin) kept at -20 degrees C. DLE stabilized with albumin and stored for 12 months at 4 degrees C inhibited the penetration of salmonellae into the liver and spleen, and their colonization in the gastrointestinal tract was significantly reduced.
本研究对从小牛肠系膜淋巴结制备并用牛白蛋白稳定化的沙门氏菌特异性可透析白细胞提取物（DLE）的活性进行了研究。评估了环境温度和储存期对DLE活性的影响。通过毛细管白细胞迁移抑制（LMI）试验检测DLE活性表明，用白蛋白稳定化的DLE在4℃储存12个月后仍保留其60%的活性。在-20℃保存的天然DLE（无白蛋白）中也保留了这一活性水平。用白蛋白稳定化并在4℃储存12个月的DLE抑制了沙门氏菌向肝脏和脾脏的侵袭，并且其在胃肠道中的定植显著减少。
