Chun J, Blackall L L, Kang S O, Hah Y C, Goodfellow M
Department of Microbiology, College of Natural Sciences, Seoul National University, Republic of Korea.
Int J Syst Bacteriol. 1997 Jan;47(1):127-31. doi: 10.1099/00207713-47-1-127.
The type strain of Nocardia pinensis was the subject of chemotaxonomic and 16S ribosomal DNA sequencing studies. The resultant nucleotide sequence was aligned with the sequences of representatives of the genera Corynebacterium, Dietzia, Gordona, Mycobacterium, Nocardia, Rhodococcus, and Tsukamurella, and phylogenetic trees were generated by using the Fitch-Margoliash, maximum-parsimony, maximum-likelihood, and neighbor-joining methods. It was evident from the phylogenetic analyses that N. pinensis represents a distinct phyletic line that is most closely associated with the Gordona clade. This genealogical evidence, together with chemotaxonomic and phenotypic data derived from this and previous studies, indicates that N. pinensis merits generic status within the family Nocardiaceae. Therefore, we propose that N. pinensis Blackall et al. 1989 be reclassified as Skermania piniformis gen. nov., comb. nov. The type strain of Skermania piniformis cleaved an array of conjugated substrates based on the fluorophores 7-amino-4-methylcoumarin and 4-methylumbelliferone.
松树诺卡氏菌的模式菌株是化学分类学和16S核糖体DNA测序研究的对象。将所得核苷酸序列与棒杆菌属、迪茨氏菌属、戈登氏菌属、分枝杆菌属、诺卡氏菌属、红球菌属和冢村氏菌属代表的序列进行比对，并使用菲奇-马戈利亚什法、最大简约法、最大似然法和邻接法构建系统发育树。系统发育分析表明，松树诺卡氏菌代表一个独特的进化谱系，与戈登氏菌分支关系最为密切。这一谱系证据，连同本研究及以往研究得出的化学分类学和表型数据，表明松树诺卡氏菌在诺卡氏菌科内值得拥有属的地位。因此，我们提议将1989年的松树诺卡氏菌Blackall等人重新分类为松树斯克尔曼氏菌新属，新组合。松树斯克尔曼氏菌的模式菌株基于荧光团7-氨基-4-甲基香豆素和4-甲基伞形酮切割一系列共轭底物。
