Yassin A F, Schaal K P
Institut für Medizinische Mikrobiologie und Immunologie der Universität Bonn, 53127 Bonn, Germany.
Int J Syst Evol Microbiol. 2005 May;55(Pt 3):1345-1348. doi: 10.1099/ijs.0.63529-0.
The type strain of Nocardia corynebacterioides was the subject of a polyphasic taxonomic study. The 16S rRNA gene sequence was aligned with the sequences of representatives of the genera Corynebacterium, Dietzia, Gordonia, Mycobacterium, Nocardia, Rhodococcus, Skermania, Tsukamurella and Williamsia, and phylogenetic trees were constructed by using maximum-parsimony, maximum-likelihood and neighbour-joining methods. It was evident from the phylogenetic analysis that N. corynebacterioides represents a distinct phyletic line within the genus Rhodococcus. Menaquinone analysis showed that the organism contained dihydrogenated menaquinone with eight isoprene units, MK-8(H(2)), as the major isoprenologue. The genealogical evidence, together with chemotaxonomic and phenotypic data from this and previous studies, indicates that N. corynebacterioides DSM 20151(T) (= CIP 104510(T)) should be reclassified in the genus Rhodococcus as Rhodococcus corynebacterioides comb. nov.
棒状诺卡氏菌的模式菌株是一项多相分类研究的对象。将16S rRNA基因序列与棒状杆菌属、迪茨氏菌属、戈登氏菌属、分枝杆菌属、诺卡氏菌属、红球菌属、斯克尔曼氏菌属、冢村氏菌属和威廉姆斯氏菌属代表的序列进行比对，并使用最大简约法、最大似然法和邻接法构建系统发育树。系统发育分析表明，棒状诺卡氏菌在红球菌属内代表一个独特的进化谱系。甲萘醌分析表明，该菌株含有具有八个异戊二烯单元的二氢甲萘醌MK-8(H₂)作为主要类异戊二烯醌。该谱系证据，连同本研究及以往研究的化学分类学和表型数据，表明棒状诺卡氏菌DSM 20151(T)(= CIP 104510(T))应重新分类到红球菌属，命名为红球菌棒状亚种，新组合。
