与柑橘黄龙病（黄化病）相关的两个物种——“亚洲韧皮杆菌”和“非洲韧皮杆菌”的16S/23S核糖体基因间隔区的比较
Comparison of the 16S/23S ribosomal intergenic regions of "Candidatus Liberobacter asiaticum" and "Candidatus Liberobacter africanum," the two species associated with citrus huanglongbing (greening) disease.
作者信息
Jagoueix S, Bove J M, Garnier M
机构信息
Laboratoire de Biologie Cellulaire et Moléculaire, Institut National de la Recherche Agronomique, Bordeaux, France.
出版信息
Int J Syst Bacteriol. 1997 Jan;47(1):224-7. doi: 10.1099/00207713-47-1-224.
16S/23S intergenic spacer regions from the rRNA operons of two strains of "Candidatus Liberobacter asiaticum" and one strain of "Candidatus Liberobacter africanum" were cloned and sequenced. The intergenic spacers of the two "Candidatus L. asiaticum" strains studied are identical and contain the genes for isoleucine tRNA (tRNA(Ile)) and alanine tRNA (tRNA(Ala)) separated by 11 nucleotides. The intergenic spacer of the "Candidatus L. africanum" strain contains only one tRNA gene (tRNA(Ala)). The level of homology between the intergenic spacers of the two liberobacter species is 79.46%. Ribosomal operons with 16S/23S spacer regions other than those studied might be present in the two "Candidatus Liberobacter" species.
对两株“亚洲韧皮杆菌（暂定种）”和一株“非洲韧皮杆菌（暂定种）”的核糖体RNA操纵子的16S/23S基因间隔区进行了克隆和测序。所研究的两株“亚洲韧皮杆菌（暂定种）”的基因间隔区相同，包含异亮氨酸转运RNA（tRNA(Ile)）和丙氨酸转运RNA（tRNA(Ala)）的基因，二者间隔11个核苷酸。“非洲韧皮杆菌（暂定种）”菌株的基因间隔区仅包含一个转运RNA基因（tRNA(Ala)）。两种韧皮杆菌属细菌的基因间隔区之间的同源性水平为79.46%。这两种“韧皮杆菌（暂定种）”可能存在除所研究的16S/23S间隔区之外的其他核糖体操纵子。
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