Basaras M, de las Heras B, García Bengoechea M, Gallego L, Arrese E, Cisterna R
Department of Microbiology, School of Medicine, Universidad del País Vasco, Bilbao, Spain.
Eur J Clin Microbiol Infect Dis. 1996 Nov;15(11):887-90. doi: 10.1007/BF01691225.
PCR was used to detect hepatitis C virus (HCV) RNA in serum and peripheral blood mononuclear cells (PBMCs) for evaluation of a six-month course of interferon therapy in 18 patients with histologically confirmed chronic hepatitis C. At follow-up six months after the end of therapy positive-stranded (genomic) and negative-stranded (anti-genomic, presumptive replicative intermediate) HCV RNA could be detected in PBMCs of all ten patients who either did not respond to therapy or suffered a relapse; genomic strand RNA was detected in five patients who responded but then relapsed. The study confirms that interferon therapy leads to inhibition of HCV replication but not eradication of the virus. Persistence of the virus at extrahepatic sites may explain its reactivation after cessation of interferon therapy.
采用聚合酶链反应（PCR）检测18例经组织学确诊为慢性丙型肝炎患者血清及外周血单个核细胞（PBMC）中的丙型肝炎病毒（HCV）RNA，以评估为期6个月的干扰素治疗疗程。在治疗结束后6个月的随访中，在所有10例对治疗无反应或复发的患者的PBMC中均可检测到正链（基因组）和负链（反基因组，推测为复制中间体）HCV RNA；在5例有反应但随后复发的患者中检测到基因组链RNA。该研究证实，干扰素治疗可导致HCV复制受到抑制，但不能根除病毒。肝外部位病毒的持续存在可能解释了干扰素治疗停止后病毒的重新激活。
