单细胞蓝藻菌株中基因组DNA的高度重复序列及特征

Highly repetitive sequences and characteristics of genomic DNA in unicellular cyanobacterial strains.

作者信息

Asayama M, Kabasawa M, Takahashi I, Aida T, Shirai M

机构信息

Division of Biotechnology, School of Agriculture, Ibaraki University, Japan.

出版信息

FEMS Microbiol Lett. 1996 Apr 1;137(2-3):175-81. doi: 10.1111/j.1574-6968.1996.tb08102.x.

DOI:10.1111/j.1574-6968.1996.tb08102.x

PMID:8998982

Abstract

Microcystis aeruginosa (Synechocystis) is a unicellular cyanobacterium that performs oxygenic photosynthesis. We found two novel sets of repetitive sequences, A (REP-A) and B (REP-B), on the M. aeruginosa K-81 genomic DNA, which consisted of distinct motifs of tandem repeated sequences located in the up- and downstream regions of the orf1 structural gene, respectively. Genomic Southern hybridization revealed multicopies of REP-A and -B on the genome. Furthermore, genomic Southern blots of cyanobacteria species with the REP-A and -B probes revealed that different hybridization signals appeared on the genomic DNAs of all 12 Microcystis strains, but no signal appeared on those of Synechocystis sp. PCC 6803, Synechococcus sp. PCC 7942, and Anabaena sp. PCC 7120.

摘要

铜绿微囊藻（集胞藻）是一种进行产氧光合作用的单细胞蓝细菌。我们在铜绿微囊藻K-81基因组DNA上发现了两组新的重复序列，A（REP-A）和B（REP-B），它们分别由位于orf1结构基因上下游区域的串联重复序列的不同基序组成。基因组Southern杂交显示基因组上存在REP-A和-B的多拷贝。此外，用REP-A和-B探针进行的蓝细菌物种基因组Southern印迹显示，在所有12株微囊藻菌株的基因组DNA上出现了不同的杂交信号，但在集胞藻属PCC 6803、聚球藻属PCC 7942和鱼腥藻属PCC 7120的基因组DNA上没有信号出现。