Garle M, Ocka R, Palonek E, Björkhem I
Department of Medical Laboratory Science and Technology, Karolinska Institute, Huddinge University Hospital, Sweden.
J Chromatogr B Biomed Appl. 1996 Dec 6;687(1):55-9. doi: 10.1016/s0378-4347(96)00210-1.
In connection with a national anti-doping control program, including analysis of 8946 urine samples, 28 athletes were found to have delivered samples free from xenobiotic anabolic steroids but with an increased testosterone/epitestosterone (T/E) ratio (> 6). Unannounced testing of the above athletes produced 2-4 additional urine samples during the next 2-3 months. A low degree of variation of the T/E ratio, with a C.V. below 30% was found in 17 of the subjects whereas 10 had a C.V. varying from 31% to 43%. One subject with a high urinary T/E ratio (10.5) had a C.V. of this ratio of 126% and also an extremely high ratio between testosterone and LH in urine. It has been reported that non-users of testosterone have T/E ratios fluctuating around a mean with a C.V. that will not exceed 30%. We found that administration of testosterone to seven healthy volunteers resulted in urinary T/E ratios that varied with a C.V. ranging from 67% to 130% during the following 4 weeks. It is concluded that among the above 28 cases, only one can be regarded as a clear case of testosterone doping. Although the vast majority of Swedish athletes have urinary T/E ratios below six, there is a subfraction with a constant higher ratio, possibly due to genetic factors.
在一项全国反兴奋剂控制计划中，对8946份尿样进行了分析，发现28名运动员的尿样中不含外源性合成代谢类固醇，但睾酮/表睾酮（T/E）比值升高（>6）。在接下来的2至3个月内，对上述运动员进行了突击检测，又采集到2至4份尿样。17名受试者的T/E比值变异程度较低，变异系数（C.V.）低于30%，而另外10名受试者的变异系数在31%至43%之间。一名尿T/E比值较高（10.5）的受试者，该比值的变异系数为126%，且尿中睾酮与促黄体生成素（LH）的比值极高。据报道，未使用睾酮的人的T/E比值围绕均值波动，变异系数不超过30%。我们发现，给7名健康志愿者服用睾酮后，在接下来的4周内，尿T/E比值的变异系数在67%至130%之间。结论是，在上述28例病例中，只有1例可被视为明确的睾酮兴奋剂使用案例。尽管绝大多数瑞典运动员的尿T/E比值低于6，但仍有一小部分人的比值持续较高，这可能是由遗传因素导致的。
