Spener F, Meusel M, Siegmann-Thoss C
Institut für Chemo-und Biosensorik, Münster, Germany.
EXS. 1997;81:27-44. doi: 10.1007/978-3-0348-9045-8_3.
When combined with biosensors as the sensing element microdialysis and flow injection analysis (FIA) systems become sophisticated tools for handling analytical processes. In particular a FIA system offers a high degree of automation together with high reproducibility and small sample volumes, whereas the biosensor, allows selective and sensitive measurements of the various analytes. Here we describe first a miniaturised microdialysis flow-through system developed for glucose determination, then we focus on amperometric immunosensors and on microbial sensors. In the former, antibodies against low molecular weight environmental contaminants or against high molecular weight proteins are responsible for analyte detection, whereas the latter use immobilised microorganisms as the recognising element for monitoring water pollutants.
当与生物传感器结合作为传感元件时，微透析和流动注射分析（FIA）系统成为处理分析过程的精密工具。特别是，FIA系统具有高度的自动化、高重现性和小样本量，而生物传感器则能够对各种分析物进行选择性和灵敏的测量。在此，我们首先描述一种为测定葡萄糖而开发的小型化微透析流通系统，然后重点介绍安培免疫传感器和微生物传感器。在前者中，针对低分子量环境污染物或高分子量蛋白质的抗体负责检测分析物，而后者则使用固定化微生物作为识别元件来监测水中污染物。
