Gwinnett A J, Tay F R, Pang K M, Wei S H
Department of Oral Biology and Pathology, School of Dental Medicine, SUNY at Stony Brook 11794-8702, USA.
Am J Dent. 1996 Aug;9(4):140-4.
To determine the quantitative contribution of dentin hybridization to bonded assembly strength and demonstrate the micromorphology of the interface with and without collagen present.
Four groups of 10 molar teeth were finished to a 320 grit dentin smear layer. Two groups served as controls and two experimental groups were subjected to collagenase digestion of the collagen exposed by acid conditioning. All-Bond 2 and Amalgambond were used to bond Bisfil and Epic resin composite, respectively. Stored in water at 37 degrees C for 24 hours the assemblies were tested in a shear mode at a crosshead speed of 5 mm/minute. Means and standard deviations were subjected to analysis for statistical significance. Twenty four teeth in four groups were examined by scanning (SEM) and transmission electron microscopy (TEM) for the relationship between resin and conditioned dentin with and without the collagen network.
All-Bond 2 and Amalgambond controls were 28.41 +/- 3.9 and 19.04 +/- 5.96 MPa, collagenase-treated groups scored 26.43 +/- 2.90 and 19.70 +/- 4.25 MPa respectively. No significant difference existed between the control and experimental groups. SEM showed an intertubular collagen network with patent tubules and a pronounced porous, irregular dentin topography following collagen digestion. A distinct hybrid zone and tubular penetration was observed but the collagenase-treated specimens showed only resin in the tubules and their lateral extensions. TEM confirmed the absence of a distinct hybrid zone in the collagenase groups with a tight, gap-free junction between the resin and the undemineralized dentin. An electron dense zone (< 50 nm) at the leading edge of conditioning was observed for All-Bond 2 and Amalgambond groups. It was concluded that the resin-reinforced or hybridized, collagenous network does not detract from, nor contribute any significant quantitative value per se to dentin bonding with the systems tested.
确定牙本质杂交对粘结组装强度的定量贡献，并展示存在和不存在胶原蛋白时界面的微观形态。
将四组每组10颗磨牙打磨至320目牙本质玷污层。两组作为对照组，两组实验组对经酸处理暴露的胶原蛋白进行胶原酶消化。分别使用全粘结2和银汞粘结剂粘结Bisfil和Epic树脂复合材料。组装体在37℃水中储存24小时后，以5毫米/分钟的十字头速度进行剪切模式测试。对平均值和标准差进行统计学显著性分析。通过扫描电子显微镜（SEM）和透射电子显微镜（TEM）检查四组中的24颗牙齿，以观察存在和不存在胶原网络时树脂与处理过的牙本质之间的关系。
全粘结2和银汞粘结剂对照组的剪切强度分别为28.41±3.9和19.04±5.96兆帕，胶原酶处理组分别为26.43±2.90和19.70±4.25兆帕。对照组和实验组之间无显著差异。SEM显示管间胶原网络，管腔开放，胶原消化后牙本质表面有明显的多孔、不规则形貌。观察到一个明显的混合区和管内渗透，但胶原酶处理的标本在管腔及其侧向延伸处仅显示树脂。TEM证实胶原酶处理组不存在明显的混合区，树脂与未脱矿牙本质之间有紧密、无间隙的连接。在全粘结2和银汞粘结剂组的处理前沿观察到一个电子致密区（<50纳米）。得出的结论是，树脂增强或杂交的胶原网络对所测试系统与牙本质的粘结既没有负面影响，本身也没有贡献任何显著的定量值。
