Ma W J, Furneaux H
Program in Molecular Pharmacology and Therapeutics, Memorial Sloan Kettering Cancer Center, New York, NY 10021, USA.
Hum Genet. 1997 Jan;99(1):32-3. doi: 10.1007/s004390050305.
The HuR gene encodes a specific RNA binding protein that is a member of the human Elav-like gene family. This family of proteins, which includes HuD, HuC and Hel-N1, is involved in cellular differentiation. Alterations of HuD and Hel-N1 structure are associated with small cell lung tumors and medulloblastomas. To investigate a possible linkage of the HuR gene to malignancy, the locus of the gene was mapped on human metaphase chromosomes. Analysis of the fluorescence signals on banded chromosomes showed that the HuR gene is localized to human chromosome 19p13.2 the cell cycle and proliferation (Levine et al. 1993; Gao et al. 1994; Liu et al. 1995; Chung et al. 1996). HuD and Hel-N1 are aberrantly spliced in human tumors yielding isoforms that are incapable of inducing differentiation (Gao et al. 1994; Antic et al. 1996). Thus it is feasible that rearrangements of the HuR gene may occur in other human tumors. In order to investigate this possibility we have mapped the HuR gene using fluorescence in-situ hybridization (FISH).
HuR基因编码一种特定的RNA结合蛋白，它是人类Elav样基因家族的成员。这个蛋白质家族包括HuD、HuC和Hel-N1，参与细胞分化。HuD和Hel-N1结构的改变与小细胞肺癌和髓母细胞瘤有关。为了研究HuR基因与恶性肿瘤之间可能的联系，该基因的位点被定位在人类中期染色体上。对带型染色体上荧光信号的分析表明，HuR基因定位于人类染色体19p13.2 细胞周期和增殖（Levine等人，1993年；Gao等人，1994年；Liu等人，1995年；Chung等人，1996年）。HuD和Hel-N1在人类肿瘤中异常剪接，产生无法诱导分化的异构体（Gao等人，1994年；Antic等人，1996年）。因此，HuR基因重排在其他人类肿瘤中可能发生是可行的。为了研究这种可能性，我们使用荧光原位杂交（FISH）对HuR基因进行了定位。
