Van Tine B A, Knops J F, Butler A, Deloukas P, Shaw G M, King P H
Department of Pathology, University of Alabama at Birmingham, Birmingham, Alabama, 35294, USA.
Genomics. 1998 Nov 1;53(3):296-9. doi: 10.1006/geno.1998.5468.
HuC is a neural-specific member of the Elav family of RNA-binding proteins. This highly conserved gene family plays a crucial role in neurogenesis, and HuC (HGMW-approved symbol ELAVL3) is expressed at an early stage of neural development. Using a novel tyramide fluorescence in situ hybridization (T-FISH) technique, we localized HuC to chromosome 19p13.2. This localization was confirmed by radiation hybrid mapping and coincides with that of HuR (HGMW-approved symbol ELAVL1), another elav family member. Dual T-FISH analysis with HuC and HuR probes, however, indicated distinct loci, with HuC being centromeric to HuR. This study demonstrates the utility of T-FISH in colocalizing two genes on the same chromosomal preparation using only biotinylated probes.
HuC是RNA结合蛋白Elav家族的神经特异性成员。这个高度保守的基因家族在神经发生中起关键作用，并且HuC（HGMW认可符号ELAVL3）在神经发育的早期阶段表达。使用一种新型酪胺荧光原位杂交（T-FISH）技术，我们将HuC定位到19号染色体p13.2区域。通过辐射杂种图谱分析证实了这一定位，并且它与另一个elav家族成员HuR（HGMW认可符号ELAVL1）的定位一致。然而，用HuC和HuR探针进行的双重T-FISH分析表明它们位于不同的位点，HuC位于HuR的着丝粒侧。这项研究证明了T-FISH仅使用生物素化探针在同一染色体标本上对两个基因进行共定位的实用性。
