Muresu R, Baldini A, Gress T, Posner J B, Furneaux H M, Siniscalco M
Porto Conte Research and Training Laboratories, Istituto di Genetica Molecolare del CNR, Alghero, Italy.
Cytogenet Cell Genet. 1994;65(3):177-8. doi: 10.1159/000133626.
A cDNA clone coding for a human brain protein (HuD), which is highly homologous to the Drosophila neuronal determination protein Elav and elicits antibody formation in a high proportion of patients with paraneoplastic encephalomyelitis, was used to isolate a lambda phage recombinant clone, including a large fragment of the relevant human genomic region. The fragment proved to be an efficient probe for the precise subregional mapping of the gene by molecular in situ hybridization onto extended human prometaphase chromosomes. Analysis of banded metaphases with clear hybridization signals pointed unequivocally to the localization of the HuD gene to human chromosome band 1p34.
一个编码人类脑蛋白（HuD）的cDNA克隆被用于分离一个λ噬菌体重组克隆，该克隆包含相关人类基因组区域的一个大片段。HuD与果蝇神经元决定蛋白Elav高度同源，并且在高比例的副肿瘤性脑脊髓炎患者中引发抗体形成。通过分子原位杂交将该片段应用于伸展的人类早中期染色体上，结果证明它是用于该基因精确亚区域定位的有效探针。对具有清晰杂交信号的带型中期染色体的分析明确指出HuD基因定位于人类染色体带1p34。
