聚合酶链反应（PCR）与杂交捕获法在检测感染人类免疫缺陷病毒高风险的注射吸毒女性中人类乳头瘤病毒的比较
Comparison of PCR and hybrid capture methods for detection of human papillomavirus in injection drug-using women at high risk of human immunodeficiency virus infection.
作者信息
Shah K V, Solomon L, Daniel R, Cohn S, Vlahov D
机构信息
Department of Molecular Microbiology and Immunology, Johns Hopkins University School of Public Health, Baltimore, Maryland 21205, USA.
出版信息
J Clin Microbiol. 1997 Feb;35(2):517-9. doi: 10.1128/jcm.35.2.517-519.1997.
Abstract
We compared Hybrid Capture, a new technique for detection of human papillomaviruses (HPV), with a PCR assay based on L1 consensus primers. By both methods, the HPV prevalence was higher in human immunodeficiency virus (HIV)-positive women than in HIV-negative women. PCR had a higher sensitivity (0.89 versus 0.48) but lower specificity (0.43 versus 0.93) for detection of Pap smear abnormalities, compared to Hybrid Capture. The higher intensity of hybridization signal by PCR was related to higher estimates of viral load by Hybrid Capture.
摘要
我们将一种用于检测人乳头瘤病毒（HPV）的新技术——杂交捕获法，与基于L1共有引物的聚合酶链反应（PCR）检测法进行了比较。通过这两种方法均发现，人类免疫缺陷病毒（HIV）阳性女性中的HPV感染率高于HIV阴性女性。与杂交捕获法相比，PCR检测巴氏涂片异常的灵敏度更高（分别为0.89和0.48），但特异性更低（分别为0.43和0.93）。PCR杂交信号强度更高与杂交捕获法对病毒载量的更高估计值相关。
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本文引用的文献
1
Persistence of type-specific human papillomavirus infection among cytologically normal women.细胞学正常女性中特定类型人乳头瘤病毒感染的持续情况。
J Infect Dis. 1994 Feb;169(2):235-40. doi: 10.1093/infdis/169.2.235.
2
Risk factors for squamous intraepithelial lesions on Pap smear in women at risk for human immunodeficiency virus infection.人类免疫缺陷病毒感染风险女性巴氏涂片检查中鳞状上皮内病变的危险因素。
J Infect Dis. 1994 Dec;170(6):1404-9. doi: 10.1093/infdis/170.6.1404.
3
Human papillomavirus testing by hybrid capture appears to be useful in triaging women with a cytologic diagnosis of atypical squamous cells of undetermined significance.通过杂交捕获法进行人乳头瘤病毒检测，对于对意义不明确的非典型鳞状细胞进行细胞学诊断的女性分流似乎是有用的。
Am J Obstet Gynecol. 1995 Mar;172(3):946-54. doi: 10.1016/0002-9378(95)90026-8.
4
Comparison of management algorithms for the evaluation of women with low-grade cytologic abnormalities.低级别细胞学异常女性评估管理算法的比较
Obstet Gynecol. 1995 Feb;85(2):202-10. doi: 10.1016/0029-7844(94)00373-L.
5
Human papillomavirus testing in primary cervical screening.原发性宫颈癌筛查中的人乳头瘤病毒检测
Lancet. 1995 Jun 17;345(8964):1533-6. doi: 10.1016/s0140-6736(95)91086-7.
6
Prevalence of human papillomavirus in cervical cancer: a worldwide perspective. International biological study on cervical cancer (IBSCC) Study Group.宫颈癌中人乳头瘤病毒的流行情况：全球视角。国际宫颈癌生物学研究（IBSCC）研究小组。
J Natl Cancer Inst. 1995 Jun 7;87(11):796-802. doi: 10.1093/jnci/87.11.796.
7
Temporal trends of incident human immunodeficiency virus infection in a cohort of injecting drug users in Baltimore, Md.马里兰州巴尔的摩市一组注射吸毒者中人类免疫缺陷病毒感染发生率的时间趋势
Arch Intern Med. 1995 Jun 26;155(12):1305-11.
8
An evaluation of human papillomavirus testing for intermediate- and high-risk types as triage before colposcopy.人乳头瘤病毒中高危型检测用于阴道镜检查前分流的评估。
Am J Obstet Gynecol. 1995 Apr;172(4 Pt 1):1150-5; discussion 1155-7. doi: 10.1016/0002-9378(95)91473-0.
9
Seroepidemiology of hepatitis B virus in a population of injecting drug users. Association with drug injection patterns.注射吸毒人群中乙型肝炎病毒的血清流行病学。与吸毒注射方式的关联。
Am J Epidemiol. 1995 Aug 1;142(3):331-41. doi: 10.1093/oxfordjournals.aje.a117639.
10
Correlates of hepatitis C virus infections among injection drug users.注射吸毒者中丙型肝炎病毒感染的相关因素
Medicine (Baltimore). 1995 Jul;74(4):212-20. doi: 10.1097/00005792-199507000-00005.
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