Koguchi A, Chiba T, Hiraga M, Hasuta Y, Tsujimoto T, Furusato K, Goryo M, Davis W C, Aida Y, Okada K
Department of Veterinary Pathology, Faculty of Agriculture, Iwate University, Morioka, Japan.
J Comp Pathol. 1996 Nov;115(4):343-52. doi: 10.1016/s0021-9975(96)80070-3.
A monoclonal antibody (MAb), c143, that recognizes a tumour-associated antigen that is "upregulated" on neoplastic B cells in cattle with enzootic bovine leukosis (EBL), was used as a marker to study disease progression. An immunohistochemical examination of neoplastic tissue and superficial cervical lymph nodes from 14 animals with EBL revealed three morphologically definable stages of change in the structure of lymph nodes, associated with the distribution of c143-positive cells: (1) the presence of c143-positive cells at the marginal sinus with no apparent changes in lymph node structure; (2) the presence of positive cells extending into and distorting the architecture of the lymph node, with clear evidence of proliferation before overt changes (enlargement of lymph nodes) were evident; and (3) the presence of positive cells throughout the lymph node with total disruption of lymph node structure when clinical signs of lymph node enlargement were evident. The results indicated that the bovine leukaemia virus-transformed lymphocytes or neoplastic cells in peripheral blood accumulate in the marginal sinus area at the earliest stages, and subsequently proliferate and infiltrate into follicles, leading to the development of clinical signs of lymphosarcoma.
一种单克隆抗体（MAb）c143，可识别一种肿瘤相关抗原，该抗原在患地方流行性牛白血病（EBL）的牛的肿瘤性B细胞上“上调”，被用作研究疾病进展的标志物。对14只患有EBL的动物的肿瘤组织和颈浅淋巴结进行免疫组织化学检查，发现淋巴结结构的变化在形态学上可分为三个阶段，这与c143阳性细胞的分布有关：（1）边缘窦处存在c143阳性细胞，淋巴结结构无明显变化；（2）阳性细胞延伸至淋巴结结构并使其变形，在明显的变化（淋巴结肿大）出现之前就有明显的增殖迹象；（3）当淋巴结肿大的临床症状明显时，整个淋巴结都有阳性细胞，淋巴结结构完全破坏。结果表明，牛白血病病毒转化的淋巴细胞或外周血中的肿瘤细胞最早在边缘窦区域积聚，随后增殖并浸润到滤泡中，导致淋巴肉瘤临床症状的出现。
