Sánchez F, Rubio M, Hernández V, Santos M, Carretero J, Vázquez R J, Vázquez R
Department of Human Anatomy and Histology, Faculty of Medicine, University of Salamanca, Spain.
Neuropeptides. 1996 Dec;30(6):515-20. doi: 10.1016/s0143-4179(96)90032-3.
In order to investigate novel neuroendocrine functions of the nitric oxide synthesizing enzyme a combined histochemical and immunocytochemical study focused on the paraventricular nucleus of the hypothalamus was conducted to check a possible influence of bilateral adrenalectomy on three different neuronal populations, NADPH diaphorase (ND)-positive, vasopressin (VP)-immunoreactive and neurons expressing both markers. In the adrenalectomized animals, a slight increase (P > 0.05) of the number of ND magnocellular neurons was detected, whereas no changes were observed in the ND-parvicellular population and in the neurons showing coexistence (magno- and parvicellular) (P > 0.05). By contrast, following bilateral adrenalectomy, a significant increase (P < 0.05) in the VP-parvicellular population (anterior, medial and periventricular subdivisions) was detected, which was reversed when the animals received daily doses of corticosterone. These results suggest that nitric oxide is not closely related to the hypothalamic regulation of the adenocorticotropin secretion exerted by the paraventricular nucleus.
为了研究一氧化氮合成酶的新型神经内分泌功能，开展了一项结合组织化学和免疫细胞化学的研究，该研究聚焦于下丘脑室旁核，以检查双侧肾上腺切除术对三种不同神经元群体（即还原型辅酶II黄递酶（ND）阳性、加压素（VP）免疫反应性以及同时表达这两种标志物的神经元）的可能影响。在肾上腺切除的动物中，检测到ND大细胞神经元数量略有增加（P>0.05），而在ND小细胞群体以及显示共存（大细胞和小细胞）的神经元中未观察到变化（P>0.05）。相比之下，双侧肾上腺切除后，检测到VP小细胞群体（前部、内侧和室周亚区）显著增加（P<0.05），当动物每日接受皮质酮剂量时，这种增加会逆转。这些结果表明，一氧化氮与室旁核对促肾上腺皮质激素分泌的下丘脑调节没有密切关系。
