Thale A B, Gordes R S, Rochels R, Tillmann B
Anatomisches Institut, Universität Kiel.
Ophthalmologe. 1996 Oct;93(5):586-91. doi: 10.1007/s003470050044.
The aim of this study was to analyze the localization and distribution of extracellular matrix in normal and glaucomatous damaged optic discs using immunohistochemical methods. Five eyes donated for corneal allografting without any history of glaucoma and three other eyes with secondary glaucoma were studied. Immunohistochemical reactions were performed with antibodies against collagen types I, III, IV and VI and against laminin, proliferating antigen KI67 and GFAP. In glaucomatous eyes the characteristic arrangement of collagen fibrils is lacking. Septa of the lamina cribrosa appear enlarged. The immunoreactivity of all examined collagen types is stronger in glaucomatous eyes. The axon basement membranes show an irregular and interrupted pattern. The number of proliferating cells with positive GFAP staining in glaucomatous cribriform plates is distinctly higher. We postulate that fibroblasts and astrocytes in the stroma of glaucomatous lamina cribrosa could be stimulated to increased and uncontrolled proliferation. Associated disorganization and raised secretion of extracellular matrix may lead to axon constriction and, secondarily, to neural degeneration.
本研究的目的是采用免疫组织化学方法分析正常和青光眼性损伤视神经盘中细胞外基质的定位和分布。研究了5只用于角膜移植且无青光眼病史的供体眼以及另外3只继发性青光眼眼。使用针对I、III、IV和VI型胶原蛋白、层粘连蛋白、增殖抗原KI67和GFAP的抗体进行免疫组织化学反应。在青光眼眼中，缺乏胶原纤维的特征性排列。筛板的间隔似乎增大。在青光眼眼中，所有检测的胶原类型的免疫反应性更强。轴突基底膜呈现不规则和中断的模式。青光眼筛板中GFAP染色阳性的增殖细胞数量明显更高。我们推测青光眼性筛板基质中的成纤维细胞和星形胶质细胞可能受到刺激而过度增殖且不受控制。相关的细胞外基质紊乱和分泌增加可能导致轴突收缩，继而导致神经变性。
