Williams B S, Doyle M D
Muritech Inc., Cambridge, MA 02139, USA.
Comput Med Imaging Graph. 1996 Nov-Dec;20(6):433-47. doi: 10.1016/s0895-6111(96)00041-9.
A prototype two- and three-dimensional color atlas of mouse development is described. The prototype has been developed using two embryos, a 13.5 d normal mouse embryo and a PATCH mutant embryo of the same age. Serial sections of the embryos, with an external registration marker system, introduced into the paraffin embedding process, were prepared by standard histological methods. For the 2D atlas, color images were digitized from 100 consecutive sections of the normal embryo. For the 3D atlas, 300 gray scale images digitized from the mutant embryo were conformally warped and reconstructed into a 3D volume dataset. The external fiducial system facilitated the three-dimensional reconstruction by providing accurate registration of consecutive images and also allowed for precise spatial calibration and the correction for warping artifacts. The atlases, with their associated anatomical knowledge base, will be integrated into a multimedia on-line information resource via the Internet's World Wide Web (WWW) using an enhanced (patent pending, Eòlas Technologies) version of the Mosaic WWW browser program from the National Center for Supercomputer Applications. These programs will provide research biologists with a set of advanced tools to analyze normal and abnormal development.
本文描述了一个小鼠发育的二维和三维彩色图谱原型。该原型是利用两个胚胎制作而成，一个是13.5天的正常小鼠胚胎，另一个是同龄的PATCH突变胚胎。通过标准组织学方法制备胚胎的连续切片，并在石蜡包埋过程中引入外部配准标记系统。对于二维图谱，从正常胚胎的100个连续切片中数字化彩色图像。对于三维图谱，从突变胚胎中数字化的300个灰度图像经过共形变形后重建为三维体积数据集。外部基准系统通过提供连续图像的精确配准促进了三维重建，还允许进行精确的空间校准和对变形伪影的校正。这些图谱及其相关的解剖学知识库，将通过互联网的万维网（WWW），使用美国国家超级计算机应用中心的增强版（专利申请中，Eòlas Technologies）Mosaic WWW浏览器程序，整合到一个多媒体在线信息资源中。这些程序将为研究生物学家提供一套先进的工具，用于分析正常和异常发育。
