多药耐药相关蛋白（MRP）基因在结直肠癌中的表达。
Expression of the multidrug resistance-associated protein (MRP) gene in colorectal carcinomas.
作者信息
Fillpits M, Suchomel R W, Dekan G, Stiglbauer W, Haider K, Depisch D, Pirker R
机构信息
Department of Oncology, University of Vienna Medical School, Austria.
出版信息
Br J Cancer. 1997;75(2):208-12. doi: 10.1038/bjc.1997.35.
To determine the clinical significance of MRP in patients with colorectal carcinomas, we have studied the expression of the MRP gene by reverse transcription-polymerase chain reaction (RT-PCR) (n = 105) and by immunohistochemistry (n = 30). MRP mRNA expression was observed in 92 (88%) tumour specimens. Positive MRP staining with monoclonal antibodies QCRL-1 and QCRL-3 was detected in all samples studied with strong staining in seven (23%) and weak staining in 23 (77%) specimens. Strong MRP staining in these samples did not appear to be related to the age and sex of the patients, localization of the primary tumour, histological grade, tumour size, lymph node metastasis, distant metastasis and tumour stage. Strong MRP staining was not associated with MDR1 RNA or P-glycoprotein (P-gp) expression. Kaplan-Meier curves revealed that overall survival of patients with strong MRP-staining tumours was similar to the survival of patients with weak-staining tumours. These data indicate that the MRP gene is expressed in primary colorectal carcinomas but is neither related to known prognostic factors nor a prognostic factor by itself.
为了确定多药耐药相关蛋白（MRP）在结直肠癌患者中的临床意义，我们通过逆转录聚合酶链反应（RT-PCR）（n = 105）和免疫组织化学（n = 30）研究了MRP基因的表达。在92个（88%）肿瘤标本中观察到MRP mRNA表达。在用单克隆抗体QCRL-1和QCRL-3进行研究的所有样本中均检测到MRP阳性染色，其中7个（23%）标本为强染色，23个（77%）标本为弱染色。这些样本中的强MRP染色似乎与患者的年龄、性别、原发肿瘤的定位、组织学分级、肿瘤大小、淋巴结转移、远处转移及肿瘤分期无关。强MRP染色与多药耐药基因1（MDR1）RNA或P-糖蛋白（P-gp）表达无关。Kaplan-Meier曲线显示，MRP强染色肿瘤患者的总生存率与弱染色肿瘤患者的生存率相似。这些数据表明，MRP基因在原发性结直肠癌中表达，但既不与已知的预后因素相关，其本身也不是一个预后因素。
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