Quílez J, Sánchez-Acedo C, Clavel A, del Cacho E, López-Bernad F
Department of Animal Pathology, Parasitology and Parasitic Diseases, Faculty of Veterinary Sciences, University Autónoma of Barcelona, Bellaterra, Spain.
Vet Parasitol. 1996 Dec 2;67(1-2):83-8. doi: 10.1016/s0304-4017(96)01026-6.
Faecal samples from 620 pigs randomly selected from 27 farms throughout Aragón were examined to determine the prevalence of Cryptosporidium infections. Detection of oocysts was performed using the ethyl-acetate stool concentration method and the modified Ziehl-Neelsen technique. Cryptosporidium parvum oocysts were identified in 136 (21.9%) pigs from 21 (77.8%) farms. Infected animals ranged from 1 to 6 months old and oocysts were not detected in suckling piglets or adults. Infection rates were significantly higher in weaned, 1-2 month old piglets (59.2%) than in fattening, 2-6 month old pigs (34.3%) (P < 0.001). Cryptosporidial infections were asymptomatic in most of the pigs (90.4%) and usually of low intensity, since 92.6% of the infected pigs excreted few oocysts (0-1 oocysts per field at x 200 magnifications). Although 24.1% of weaned and 5.6% of fattening pigs infected by C. parvum had diarrhoea, it was not found to be statistically associated with infection. In fact, infection rates were higher in non-diarrhoeic than in diarrhoeic pigs, in both weaned (64.7% and 46.7%, respectively) and fattening pigs (34.3% and 33.3%).
从阿拉贡地区27个农场随机选取620头猪的粪便样本进行检测，以确定隐孢子虫感染的流行情况。采用乙酸乙酯粪便浓缩法和改良齐-尼氏染色法检测卵囊。在来自21个（77.8%）农场的136头（21.9%）猪中发现了微小隐孢子虫卵囊。感染动物年龄在1至6个月之间，在哺乳仔猪或成年猪中未检测到卵囊。断奶后1至2月龄仔猪的感染率（59.2%）显著高于育肥阶段2至六个月龄猪（34.3%）（P < 0.001）。大多数猪（90.4%）的隐孢子虫感染无症状，且感染强度通常较低，因为92.6%的感染猪排出的卵囊数量较少（在200倍放大倍数下，每个视野0至1个卵囊）。虽然24.1%的断奶仔猪和5.6%的育肥猪感染微小隐孢子虫后出现腹泻，但腹泻与感染之间未发现统计学关联。事实上，在断奶仔猪（分别为64.7%和46.7%）和育肥猪（34.3%和33.3%）中，未腹泻猪的感染率高于腹泻猪。
