Riesgo-Escovar J R, Piekos W B, Carlson J R
Department of Biology, Yale University, New Haven, CT 06520-8103, USA.
J Comp Physiol A. 1997 Feb;180(2):151-60. doi: 10.1007/s003590050036.
The physiology and ultrastructure of the antenna in Drosophila melanogaster have been examined in wild-type and lozenge mutants. Scanning electron microscopy (SEM) of sensilla on the antennal surface has revealed that in the wild-type the basiconic sensilla contain linear arrays of pores connected by longitudinal furrows and transected by shorter furrows. Sensilla trichodea also are shown to have pores, as revealed by examining transverse sections by transmission electron microscopy (TEM); these data directly address a longstanding controversy. Coeloconic sensilla, previously described as "pit sensilla" and as "grooved" sensilla, are shown to rise directly from the antennal surface, as opposed to lying below the antennal surface in pits; the previously observed grooves correspond to the junctions between bundled, finger-like projections. This description of coeloconic sensilla is supported by analysis of lz mutants, in which the projections of coeloconic sensilla splay apart. Coeloconic sensilla are also shown to undergo duplication on the lz3 antenna. Physiological recordings from the antenna show that responses to all odorants tested are severely decreased in lz mutants. Measurements made from different parts of the antenna show similar defects. Evidence is provided that both the physiological and ultrastructural defects map to the lz locus.
在野生型和菱形突变体果蝇中，已对其触角的生理学和超微结构进行了研究。对触角表面感觉器的扫描电子显微镜（SEM）观察显示，在野生型中，锥形感觉器包含由纵向沟连接并被较短沟横切的线性孔阵列。通过透射电子显微镜（TEM）检查横切面发现，毛形感觉器也有孔；这些数据直接解决了一个长期存在的争议。腔锥感觉器，以前被描述为“坑状感觉器”和“有槽感觉器”，现在显示是直接从触角表面升起，而不是位于触角表面下方的坑中；先前观察到的槽对应于成束的指状突起之间的连接处。对菱形突变体（lz突变体）的分析支持了对腔锥感觉器的这种描述，在该突变体中，腔锥感觉器的突起会散开。在lz3触角上还发现腔锥感觉器会发生复制。对触角的生理学记录表明，在lz突变体中，对所有测试气味剂的反应都严重降低。从触角不同部位进行的测量显示出类似的缺陷。有证据表明，生理学和超微结构缺陷均映射到lz基因座。
