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Direct detection of equine herpesvirus DNA in tissues of aborted equine fetuses.

作者信息

Ishiyama T, Nishimori T, Kato M, Yamada H, Sato K, Sentsui H

机构信息

Hokkaido Branch, National Institute of Animal Health, Japan.

出版信息

Zentralbl Veterinarmed B. 1996 Dec;43(10):639-42. doi: 10.1111/j.1439-0450.1996.tb00363.x.

DOI:10.1111/j.1439-0450.1996.tb00363.x
PMID:9011160
Abstract

Restriction endonuclease analysis of equine herpesviruses 1 (EHV-1) and 4 has been investigated using cultured cells infected with these viruses. The DNA cleavage patterns of these viruses were observed in the intracellular DNA after digestion with Eco RI and electrophoresis. This procedure was applied to the diagnosis of equine herpesvirus infection in aborted equine fetuses. The characteristic Eco RI restriction pattern of EHV-1 DNA was directly detectable in the emulsion of lungs collected from aborted equine fetuses.

摘要

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