Sung H W, Hsu C S, Chen H C, Hsu H L, Chang Y, Lu J H, Yang P C
Department of Chemical Engineering, National Central University, Chung-Li, Taipei, Taiwan, Republic of China.
Artif Organs. 1997 Jan;21(1):50-8. doi: 10.1111/j.1525-1594.1997.tb00699.x.
The clinical results of biological vascular grafts have been unsatisfactory. The poor results of these vascular grafts may be attributed to the fixatives, aldehydes, used in fixing tissues. In an attempt to overcome this problem, a new fixative, epoxy compound, has recently been used to fix biological vascular grafts. The study was undertaken to investigate the crosslinking characteristics, fixation index and denaturation temperature, of various porcine arteries fixed with an epoxy compound. The porcine arteries investigated in the study were the common carotid artery, internal thoracic artery, abdominal aorta, and saphenous artery. In addition, the effects of sonication on the porcine arteries before fixation on their crosslinking characteristics were analyzed. The fresh and glutaraldehyde-fixed arteries were used as controls. It was noted that glycine, proline, and alanine were the most abundant amino acids found in the porcine internal thoracic artery. In the amino acid analysis, it was observed that the amino acids in the porcine arteries reacted with epoxy compound or glutaraldehyde were lysine, hydroxylysine, histidine, and arginine. Of these amino acids, lysine was the most reactive. In general, the fixed arteries were relatively stiffer than their fresh counterparts. The fixation indices and denaturation temperatures of various porcine arteries were comparable throughout the entire fixation process. The amounts of free amino groups of the sonicated arteries were significantly lower than those of their unsonicated counterparts (p < 0.05). It is speculated that the diminishing free amino groups of the sonicated arteries may be attributed to the removal of the destroyed cell debris and adherent proteins of the arteries after sonication. However, it was learned that sonication on the porcine arteries before fixation did not seem to affect their fixation indices and denaturation temperatures. The results obtained in this study may help one in selecting the raw materials for developing a small-diameter biological vascular graft
生物血管移植物的临床效果一直不尽人意。这些血管移植物效果不佳可能归因于用于固定组织的固定剂——醛类。为克服这一问题，一种新型固定剂——环氧化合物，最近已被用于固定生物血管移植物。本研究旨在调查用环氧化合物固定的各种猪动脉的交联特性、固定指数和变性温度。本研究中所调查的猪动脉包括颈总动脉、胸廓内动脉、腹主动脉和隐动脉。此外，还分析了固定前超声处理对猪动脉交联特性的影响。新鲜动脉和经戊二醛固定的动脉用作对照。值得注意的是，甘氨酸、脯氨酸和丙氨酸是在猪胸廓内动脉中发现的最丰富的氨基酸。在氨基酸分析中，观察到猪动脉中与环氧化合物或戊二醛反应的氨基酸为赖氨酸、羟赖氨酸、组氨酸和精氨酸。在这些氨基酸中，赖氨酸反应性最强。一般来说，固定后的动脉比新鲜动脉相对更硬。在整个固定过程中，各种猪动脉的固定指数和变性温度相当。超声处理后的动脉游离氨基数量明显低于未超声处理的动脉（p < 0.05）。据推测，超声处理后动脉游离氨基数量减少可能归因于超声处理后动脉中被破坏的细胞碎片和附着蛋白的去除。然而，研究发现固定前对猪动脉进行超声处理似乎并不会影响其固定指数和变性温度。本研究获得的结果可能有助于人们选择用于开发小直径生物血管移植物的原材料。
