Mure K, Hayatsu H, Takeuchi T, Takeshita T, Morimoto K
Department of Hygiene and Preventive Medicine, Osaka University School of Medicine, Japan.
Mutat Res. 1997 Jan 3;373(1):107-11. doi: 10.1016/s0027-5107(96)00195-9.
We examined the mutagenicity of cigarette smoker's urine in 32 healthy male cigarette smoker and 37 healthy male non-smoker. Twenty-four-hour urine specimens were subjected to blue rayon extraction which selectively adsorb polycyclic compounds, after which the elutions were fractionated by carboxymethyl cellulose column chromatography for removing antimutagenic compounds. The mutagens were measured by using an S9-mediated Salmonella mutagenicity test on strain TA98. Compared with those with non-smokers, smokers' urine showed a significantly higher urinary level of mutagens in the acid-elutable and in the sum of all chromatography fractions. A similar tendency was also seen in the alkali-elutable fraction. The subjects were classified into three groups according to the number of smoked cigarettes. Heavy smokers, who smoked more than 20 cigarettes per day, showed a significantly higher urinary level of mutagens than both non-smokers and light smokers especially in the acid-elutable and in the sum of all chromatography fractions. Our findings suggest that smokers are exposed to a great amount of polycyclic carcinogens and mutagens by cigarette smoking. These results also suggest that urinary level of mutagens measured by using blue rayon extraction combined with carboxymethyl cellulose chromatography could be a good index for estimating the exposure to carcinogens and mutagens such as polycyclic compounds.
我们检测了32名健康男性吸烟者和37名健康男性非吸烟者尿液的致突变性。收集24小时尿液样本，通过蓝人造丝萃取法选择性吸附多环化合物，之后将洗脱液通过羧甲基纤维素柱色谱法进行分离，以去除抗诱变化合物。使用TA98菌株的S9介导的沙门氏菌致突变性试验来测定诱变剂。与非吸烟者相比，吸烟者尿液中酸洗脱部分以及所有色谱级分总和中的诱变剂水平显著更高。在碱洗脱部分也观察到类似趋势。根据吸烟数量将研究对象分为三组。每天吸烟超过20支的重度吸烟者，其尿液中的诱变剂水平显著高于非吸烟者和轻度吸烟者，尤其是在酸洗脱部分以及所有色谱级分总和中。我们的研究结果表明，吸烟者通过吸烟接触到大量多环致癌物和诱变剂。这些结果还表明，使用蓝人造丝萃取结合羧甲基纤维素色谱法测定的尿液诱变剂水平可能是评估多环化合物等致癌物和诱变剂暴露情况的良好指标。
