Haas H, Angermayr K, Stöffler G
Institut für Mikrobiologie (Medizinische Fakultät), Universität Innsbruck, Austria.
Gene. 1997 Jan 3;184(1):33-7. doi: 10.1016/s0378-1119(96)00570-7.
Employing a PCR-aided strategy, a Penicillium chrysogenum gene (sreP) encoding a putative GATA-transcription factor has been cloned and characterized. Comparison of the genomic and cDNA sequences revealed the presence of an open reading frame (ORF) encoding a protein of 532 amino acids (aa) which is interrupted by two introns. The deduced aa sequence of sreP reveals 50% identity to a regulator of siderophore biosynthesis (URBS1) from Ustilago maydis over a stretch of 200 aa containing two GATA-type zinc finger motifs and a Cys-rich intervening sequence. Northern blot analysis indicated two transcripts of 2.2 and 2.7 kb in approximately equivalent amount. due to two major transcription start sites.
采用聚合酶链反应（PCR）辅助策略，已克隆并鉴定了产黄青霉中一个编码假定GATA转录因子的基因（sreP）。基因组序列与cDNA序列的比较显示，存在一个开放阅读框（ORF），其编码一个由532个氨基酸（aa）组成的蛋白质，该蛋白质被两个内含子打断。sreP推导的氨基酸序列与来自玉米黑粉菌的铁载体生物合成调节因子（URBS1）在一段包含两个GATA型锌指基序和一个富含半胱氨酸的中间序列的200个氨基酸上有50%的同一性。Northern印迹分析表明，由于两个主要转录起始位点，存在两种大小分别为2.2 kb和2.7 kb的转录本，其含量大致相当。
