血浆磷脂中的反油酸和反式-11-十八碳烯酸作为18:1反式脂肪酸膳食摄入量的指标。

Elaidic and trans-vaccenic acids in plasma phospholipids as indicators of dietary intake of 18:1 trans-fatty acids.

作者信息

Seppänen-Laakso T, Laakso I, Backlund P, Vanhanen H, Viikari J

机构信息

Department of Pharmacy, FIN-00014 University of Helsinki, Finland.

出版信息

J Chromatogr B Biomed Appl. 1996 Dec 13;687(2):371-8. doi: 10.1016/s0378-4347(96)00256-3.

DOI:10.1016/s0378-4347(96)00256-3

PMID:9017460

Abstract

Octadecenoic (18:1) trans-fatty acid fractions from margarine, butter and plasma phospholipids (PL) were isolated by silver ion TLC, and nine positional isomers (n-11-n-3) were identified by GC-MS based on their ozonolysis products. The GC analysis of the isolated fractions gave similar peak profiles and separated seven trans-isomers (n-11-n-6 and n-3). Without a preceding isolation step, the reproducibility of the GC method for plasma PL elaidic (18:1 n 9 trans) and trans-vaccenic acids (n-7) was 3.4 and 2.7% (R.S.D.), respectively. These trans-isomers were rapidly incorporated and cleared in plasma PL and they closely reflected both increased and decreased intake of 18:1 trans-fatty acids during moderate fat substitutions. Significant associations between high-density lipoprotein cholesterol (HDL-C) and PL elaidic and trans-vaccenic acids appeared in habitual margarine users only.

摘要

通过银离子薄层层析从人造黄油、黄油和血浆磷脂（PL）中分离出十八碳烯酸（18:1）反式脂肪酸组分，并基于其臭氧分解产物通过气相色谱 - 质谱联用仪（GC - MS）鉴定出九种位置异构体（n - 11 - n - 3）。对分离出的组分进行气相色谱分析得到了相似的峰形，并分离出七种反式异构体（n - 11 - n - 6和n - 3）。在没有先前分离步骤的情况下，气相色谱法测定血浆PL中反油酸（18:1 n 9反式）和反式vaccenic酸（n - 7）的重现性分别为3.4%和2.7%（相对标准偏差）。这些反式异构体在血浆PL中迅速被摄取和清除，并且在适度脂肪替代期间，它们密切反映了18:1反式脂肪酸摄入量的增加和减少。仅在习惯食用人造黄油的人群中，高密度脂蛋白胆固醇（HDL - C）与血浆PL中的反油酸和反式vaccenic酸之间出现了显著关联。