Kedes D H, Ganem D, Ameli N, Bacchetti P, Greenblatt R
Department of Microbiology, University of California, San Francisco 94143, USA.
JAMA. 1997 Feb 12;277(6):478-81.
To determine the prevalence of human herpesvirus 8 (HHV-8) seropositivity among women who are known to be infected with human immunodeficiency virus type 1 (HIV-1) or who are at high risk for HIV infection.
A cross-sectional and blinded study of the prevalence of serological reactivity to HHV-8 infection as determined by an indirect immunofluorescence assay using nuclei from cells latently infected with HHV-8. Data and specimens were collected at baseline assessments of a longitudinal natural history cohort study.
Four San Francisco Bay Area outpatient HIV specialty clinics participating in the cohort study.
A total of 387 participants in the Women's Interagency HIV Study whose HIV infection status was documented and serum was available in a local specimen repository.
Serological reactivity to HHV-8.
Serological reactivity to latent HHV-8 antigens was uncommon among study participants: 13 (3.4%) demonstrated serum antibodies. HHV-8 reactivity was more common among HIV-infected women; 12 (4.0%; 95% confidence interval [CI], 2.1%-6.9%) of the 302 HIV-infected participants vs 1 (1.2%; 95% CI, 0.03%-6.4%) of the 84 HIV-uninfected participants were seropositive for HHV-8, though the difference did not attain statistical significance (odds ratio=3.43; 95% CI, 0.49-148.6). Two of the HIV-infected women had Kaposi sarcoma lesions and both were seropositive.
The prevalence of HHV-8 seropositivity among the group of HIV-infected women was dramatically lower than that recently reported among HIV-infected homosexual and bisexual men (30%-35%). This finding parallels the lower prevalence of Kaposi sarcoma among women in contrast to men with HIV infection. These data further extend the correlation of HHV-8 serological reactivity with risk of Kaposi sarcoma and are consistent with an important role for HHV-8 infection in development of Kaposi sarcoma.
确定已知感染1型人类免疫缺陷病毒（HIV-1）或有HIV感染高风险的女性中人类疱疹病毒8型（HHV-8）血清阳性的患病率。
一项横断面盲法研究，采用间接免疫荧光法，以潜伏感染HHV-8的细胞的细胞核作为抗原，检测HHV-8感染的血清学反应性患病率。数据和标本收集自一项纵向自然史队列研究的基线评估。
旧金山湾区的四家参与该队列研究的门诊HIV专科诊所。
妇女机构间HIV研究的387名参与者，其HIV感染状况有记录，且血清保存在当地标本库中。
对HHV-8的血清学反应性。
研究参与者中对潜伏HHV-8抗原的血清学反应不常见：13人（3.4%）显示有血清抗体。HHV-8反应性在HIV感染女性中更常见；302名HIV感染参与者中有12人（4.0%；95%置信区间[CI]，2.1%-6.9%）HHV-8血清阳性，而84名未感染HIV的参与者中有1人（1.2%；95%CI，0.03%-6.4%）HHV-8血清阳性，尽管差异无统计学意义（优势比=3.43；95%CI，0.49-148.6）。两名HIV感染女性有卡波西肉瘤病变，且均为血清阳性。
HIV感染女性中HHV-8血清阳性的患病率显著低于最近报道的HIV感染同性恋和双性恋男性中的患病率（30%-35%）。这一发现与HIV感染女性中卡波西肉瘤患病率低于男性的情况相似。这些数据进一步扩展了HHV-8血清学反应性与卡波西肉瘤风险的相关性，并与HHV-8感染在卡波西肉瘤发生中的重要作用相一致。
