Alberti I, Parodi S, Barboro P, Sanna P, Nicolò G, Allera C, Patrone E, Galli S, Balbi C
Istituto Nazionale per la Ricerea sul Cancro, Genova, Italy.
Cancer Lett. 1996 Dec 3;109(1-2):193-8. doi: 10.1016/s0304-3835(96)04444-8.
We have characterized the changes in composition of the nuclear matrix-intermediate filament complex (NM-IF) isolated from prostate cancer (PCa), compared with benign prostatic hyperplasia (BPH). We prepared the NM-IF from ten patients undergoing radical retropubic prostectomy; the benign hyperplastic tissue was obtained from the prostate lobe contralateral to the cancer zone. Several quantitative and qualitative changes have been identified. Three new proteins of molecular weight 48, 47 and 29 kDa and isoelectric point 6.0, 4.9 and 6.4, respectively, were detected in PCa, referred to here as P8, P5 and NM-1, P8 was present in all ten of the tumors examined, P5 was expressed in 9/10 PCa; conversely, they were present in only one and two BPH, respectively; NM-1 was found in eight tumors out of nine and never in BPH. These proteins are expressed in moderately differentiated malignant cells, suggesting that the proteins of the NM-IF complex can be interesting biomarkers for prostate cancer. Immunoblot analysis shows that P8 and P5 proteins belong to the IF superfamily. This observation, taken together with previous data obtained by our and other groups, suggests that the characterization of NM-IF protein changes could also shed light on mechanistic aspects of cancer progression.
我们已经对从前列腺癌（PCa）中分离出的核基质-中间丝复合物（NM-IF）的组成变化进行了表征，并与良性前列腺增生（BPH）进行了比较。我们从10例接受耻骨后根治性前列腺切除术的患者中制备了NM-IF；良性增生组织取自癌灶对侧的前列腺叶。已确定了一些定量和定性变化。在PCa中检测到三种分子量分别为48、47和29 kDa、等电点分别为6.0、4.9和6.4的新蛋白，在这里称为P8、P5和NM-1。P8在所检查的所有10个肿瘤中均存在，P5在9/10的PCa中表达；相反，它们分别仅在1个和2个BPH中存在；NM-1在9个肿瘤中的8个中被发现，而在BPH中从未发现。这些蛋白在中度分化的恶性细胞中表达，表明NM-IF复合物的蛋白可能是前列腺癌有趣的生物标志物。免疫印迹分析表明，P8和P5蛋白属于中间丝超家族。这一观察结果与我们和其他研究小组先前获得的数据一起表明，NM-IF蛋白变化的表征也可能有助于揭示癌症进展的机制方面。
