González-Piña R, Paz C
Departamento de Neurofisiologia, Instituto Nacional de Neurologia y Neurocirugía, México, D.F.
Neurochem Res. 1997 Jan;22(1):63-6. doi: 10.1023/a:1027329405112.
We have shown in our laboratory that cat's and rat's sleep disturbances are produced by 24 h of ozone (O3) exposure, indicating that the central nervous system is affected by this gas. To demonstrate the probable changes in brain neurotransmitters, we evaluated the monoamine contents of the midbrain and striatum of rats exposed to 1 part per million O3 for 1 or 3 hours periods. The results were compared with rats exposed to fresh air and to those exposed to 3 hours of O3 followed by 1 or 3 hours of fresh air. We found a significant increase in dopamine (DA) and its metabolites noradrenaline (NA) and 3,4 dihydroxyphenylacetic acid (DOPAC), as well as an increase in the 5-hydroxyindolacetic acid (5-HIAA) contents of the striatum. There were no changes in homovanillic acid (HVA) and serotonin (5-HT) levels during O3 exposure. Additionally, an increase in DA, NA and 5-HIAA in the midbrain during O3 exposure was observed. Turnover analysis revealed that DA increased more than its metabolites in both the midbrain and striatum. However, the metabolite of 5-HT, i.e. 5-HIAA, increased more than its precursor, this reaching statistical significance only in the midbrain. These findings demonstrate that O3 or its reaction products affect the metabolism of major neurotransmitter systems as rapidly as after 1 h of exposition.
