van der Linden P J, de Goeij A F, Dunselman G A, van der Linden E P, Ramaekers F C, Evers J L
Department of Obstetrics and Gynecology, Academisch Ziekenhuis Maastricht, The Netherlands.
Fertil Steril. 1994 Jan;61(1):85-90. doi: 10.1016/s0015-0282(16)56457-7.
To detect the expression of integrins and E-cadherin in cells from peritoneal fluid (PF), endometrium, menstrual effluent, peritoneum, and endometriotic lesions during the early follicular phase of the menstrual cycle.
An immunohistochemical study.
Tertiary care university medical center.
Sixteen patients undergoing a diagnostic laparoscopy as part of a subfertility work-up. All patients had regular and ovulatory cycles.
A laparoscopy was performed in the early follicular phase (days 2 to 5). Simultaneously, samples were taken from endometrium, menstrual effluent, and PF, and a representative biopsy of an endometriotic lesion was obtained. If endometriosis was not noted, a peritoneal biopsy was obtained instead.
The expression of cell adhesion molecules, including the integrin alpha 2 beta 1, alpha 3 beta 1, alpha 4 beta 1, alpha 5 beta 1, and alpha 6 beta 1 and E-cadherin, as determined by immunohistochemistry on frozen sections.
All integrins tested could be detected in the endometrium samples and in endometriotic lesions. In menstrual effluent samples, positive staining for the integrins alpha 2 beta 1 and alpha 3 beta 1 was found in epithelial cells in 13 of 16 cases. Integrin alpha 5 beta 1 was detected in 11 of 16 samples, and integrins alpha 4 beta 1 and alpha 6 beta 1 were detected in 5 of 16 samples. In PF, integrin alpha 3 beta 1 was found in epithelial cells in 12 of 16 samples, integrin alpha 5 beta 1 in 5 of 16, and integrins alpha 4 beta 1 in 2 of 16. The antibody for E-cadherin showed positive staining of epithelial cells in 6 of 16 menstrual effluent samples. All endometrial tissue samples showed positive staining for E-cadherin. In PF, E-cadherin was detected in the epithelial cells of one sample. One peritoneum biopsy revealed positive staining for E-cadherin.
Integrins alpha 2 beta 1, alpha 3 beta 1, alpha 4 beta 1, alpha 5 beta 1, and E-cadherin, important cell adhesion molecules, are expressed in endometriotic lesions and in cells and tissues that are potentially involved in the development of endometriosis. These cell adhesion molecules could be involved in the shedding of endometrial tissue during menstruation and the attachment of endometrial tissue fragments to the peritoneum.
检测月经周期卵泡早期腹膜液（PF）、子宫内膜、月经流出物、腹膜及子宫内膜异位症病灶细胞中整合素和E-钙黏蛋白的表达。
一项免疫组织化学研究。
三级医疗大学医学中心。
16例因不孕症检查接受诊断性腹腔镜检查的患者。所有患者月经周期规律且有排卵。
在卵泡早期（第2至5天）进行腹腔镜检查。同时，采集子宫内膜、月经流出物和PF样本，并获取子宫内膜异位症病灶的代表性活检样本。若未发现子宫内膜异位症，则取腹膜活检样本。
通过对冰冻切片进行免疫组织化学检测，确定细胞黏附分子整合素α2β1、α3β1、α4β1、α5β1、α6β1和E-钙黏蛋白的表达。
所有检测的整合素均可在子宫内膜样本和子宫内膜异位症病灶中检测到。在月经流出物样本中，16例中有13例上皮细胞中整合素α2β1和α3β1呈阳性染色。16例中有11例检测到整合素α5β1，16例中有5例检测到整合素α4β1和α6β1。在PF中，16例中有12例上皮细胞中发现整合素α3β1，16例中有5例发现整合素α5β1，16例中有2例发现整合素α4β1。E-钙黏蛋白抗体在16例月经流出物样本中有6例上皮细胞呈阳性染色。所有子宫内膜组织样本E-钙黏蛋白均呈阳性染色。在PF中，一个样本的上皮细胞中检测到E-钙黏蛋白。一份腹膜活检样本E-钙黏蛋白呈阳性染色。
整合素α2β1、α3β1、α4β1、α5β1和E-钙黏蛋白这些重要的细胞黏附分子，在子宫内膜异位症病灶以及可能参与子宫内膜异位症发生发展的细胞和组织中表达。这些细胞黏附分子可能参与月经期间子宫内膜组织的脱落以及子宫内膜组织碎片附着于腹膜的过程。
