Lewis J B, Anderson C W, Atkins J F
Cell. 1977 Sep;12(1):37-44. doi: 10.1016/0092-8674(77)90183-0.
RNA isolated from the cytoplasm of human cells at late times after infection by adenovirus type 2 (Ad2) has been fractionated by hybridization to fragments of Ad2 DNA which were produced by digestion with the restriction endonucleases Hpa I, Eco RI, Bam HI and Hind III. Cell-free translation of these partially purified mRNAs indicates that the genes for the late Ad2 proteins lie within the following intervals on the conventional Ad2 map: 15K (4.4-17.0 map units), IX and IVa2 (7.5-17.0), IIIa (29.1-40.9), III and V (29.1-57.0), pVIII (40.9-57.0), pVI and II (40.9-70.7), 100K (59.0-83.4), pVIII (70.7-83.4) and IV (85.0-100). In addition to the primary hybridization of the late Ad2 mRNAs to the regions indicated above, most late Ad2 mRNAs (except those for 15K, IX and IVa2) exhibited some hybridization to a secondary site between 17.0 and 29.1 map units.
在被2型腺病毒（Ad2）感染后的晚期，从人细胞质中分离出的RNA，通过与用限制性内切酶Hpa I、Eco RI、Bam HI和Hind III消化产生的Ad2 DNA片段杂交进行了分级分离。对这些部分纯化的mRNA进行无细胞翻译表明，Ad2晚期蛋白的基因位于传统Ad2图谱上的以下区间：15K（4.4 - 17.0图谱单位）、IX和IVa2（7.5 - 17.0）、IIIa（29.1 - 40.9）、III和V（29.1 - 57.0）、pVIII（40.9 - 57.0）、pVI和II（40.9 - 70.7）、100K（59.0 - 83.4）、pVIII（70.7 - 83.4）和IV（85.0 - 100）。除了晚期Ad2 mRNA与上述区域的主要杂交外，大多数晚期Ad2 mRNA（除了15K、IX和IVa2的mRNA）还与17.0至29.1图谱单位之间的一个次要位点表现出一些杂交。
