Kruijer W, Van Schaik F M, Sussenbach J S
Nucleic Acids Res. 1982 Aug 11;10(15):4493-500. doi: 10.1093/nar/10.15.4493.
We have determined the nucleotide sequence of the gene encoding adenovirus type 2 (Ad2) DNA binding protein (DBP). From the nucleotide sequence the complete amino acid sequence of Ad2 DBP has been deduced. A comparison of the amino acid sequences of Ad2 and Ad5 DBP, both 529 residues long, reveals that the C-terminal 354 residues of both sequences are identical. Within the N-terminal 175 amino acid residues Ad2 and Ad5 show nine differences. The site of mutation in Ad2 ND1ts23, a mutant with a temperature-sensitive DNA replication, was mapped at the nucleotide level. A single nucleotide alteration in the DBP gene, resulting in a leucine leads to phenylalanine substitution at position 282 in the amino acid sequence is responsible for the temperature-sensitive character of this mutant. Previously, we localized the mutation of another DBP mutant with a temperature-sensitive DNA replication (H5ts125) at position 413 in the amino acid sequence of the DBP molecule (Nucleic Acids Res. 9 (1981) 4439-4457). These mapping data are discussed in relation to the structure and function of the DBP molecule.
我们已经确定了编码腺病毒2型（Ad2）DNA结合蛋白（DBP）的基因的核苷酸序列。从核苷酸序列推断出了Ad2 DBP的完整氨基酸序列。Ad2和Ad5 DBP的氨基酸序列长度均为529个残基，比较结果显示这两个序列的C端354个残基是相同的。在N端的175个氨基酸残基范围内，Ad2和Ad5有9处差异。对Ad2 ND1ts23（一种具有温度敏感性DNA复制的突变体）的突变位点进行了核苷酸水平的定位。DBP基因中的单个核苷酸改变，导致氨基酸序列第282位的亮氨酸被苯丙氨酸取代，这是该突变体具有温度敏感性的原因。此前，我们将另一个具有温度敏感性DNA复制的DBP突变体（H5ts125）的突变定位在DBP分子氨基酸序列的第413位（《核酸研究》9（1981）4439 - 4457）。结合DBP分子的结构和功能对这些定位数据进行了讨论。
