一种用于从基因工程苏云金芽孢杆菌菌株中消除抗生素抗性基因标记的重组酶介导系统。
A recombinase-mediated system for elimination of antibiotic resistance gene markers from genetically engineered Bacillus thuringiensis strains.
作者信息
Sanchis V, Agaisse H, Chaufaux J, Lereclus D
机构信息
Unité de Biochimie Microbienne, Institut Pasteur, URA 1300 CNRS, Paris, France.
出版信息
Appl Environ Microbiol. 1997 Feb;63(2):779-84. doi: 10.1128/aem.63.2.779-784.1997.
Abstract
A TnpI-mediated site-specific recombination system to construct genetically modified Bacillus thuringiensis strains was developed. Recombinant B. thuringiensis strains from which antibiotic resistance genes can be selectively eliminated were obtained in vivo with a new vector based on the specific resolution site of transposon Tn4430. For example, a cryIC gene, whose product is active against Spodoptera littoralis, was introduced into B. thuringiensis Kto harboring a cryIA(c) gene active against Ostrinia nubilalis. The resulting strain had a broader activity spectrum than that of the parental strain. It contained only B. thuringiensis DNA and was free of antibiotic resistance genes. This should facilitate regulatory approval for its development as a commercial biopesticide.
摘要
开发了一种用于构建转基因苏云金芽孢杆菌菌株的由转座酶抑制蛋白（TnpI）介导的位点特异性重组系统。利用基于转座子Tn4430特异性解离位点的新型载体，在体内获得了可选择性消除抗生素抗性基因的重组苏云金芽孢杆菌菌株。例如，将对棉铃虫有活性的cryIC基因导入携带对欧洲玉米螟有活性的cryIA(c)基因的苏云金芽孢杆菌Kto中。所得菌株的活性谱比亲本菌株更宽。它仅含有苏云金芽孢杆菌的DNA，且不含抗生素抗性基因。这应有助于其作为商业生物杀虫剂开发时获得监管批准。
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