Samuel L H, Anderson G, Mintz P D
Department of Pathology, University of Virginia Health Sciences Center, Charlottesville, USA.
Transfusion. 1997 Jan;37(1):25-8. doi: 10.1046/j.1537-2995.1997.37197176947.x.
Gamma irradiation of blood components is used to prevent transfusion-associated graft-versus-host disease. The demand for irradiated blood components is increasing because of the increase in directed donation by family members. Irradiated units currently have a recommended maximum storage life of 28 days. Since in vivo recovery is related to red cell ATP levels, rejuvenation of stored irradiated units using a pyruvate-inosine phosphate-adenine additive was explored.
Units of AS-1 red cells from 16 volunteer donors were divided into two equal volumes and one split unit from each was irradiated with 25 Gy. Ten units were irradiated on Day 5, 6, or 7 of 4 degrees C storage and 6 units were irradiated on Day 1 of 4 degrees C storage. All units were rejuvenated for 1 hour at 37 degrees C using a pyruvate-inosine-phosphate-adenine additive on Day 42 of 4 degrees C storage. Units were assayed for ATP, 2, 3 DPG and supernatant sodium, potassium, and glucose.
ATP and 2, 3 DPG levels were restored equally well in irradiated and non-irradiated units. The previously reported irradiation-induced red cell potassium-sodium shift was demonstrated. Supernatant potassium and sodium levels did not reverse 1 hour after rejuvenation was completed. There was no significant difference in results between units irradiated on Day 1 or Day 5, 6, or 7.
Red cell ATP and 2, 3 DPG levels were restored in irradiated AS-1 units stored at 4 degrees C for 42 days using a pyruvate-inosine-phosphate-adenine rejuvenation additive.
血液成分的γ射线辐照用于预防输血相关移植物抗宿主病。由于家庭成员定向献血的增加，对辐照血液成分的需求正在上升。目前辐照单位推荐的最长储存期为28天。由于体内恢复与红细胞ATP水平有关，因此探索了使用丙酮酸 - 肌苷磷酸 - 腺嘌呤添加剂对储存的辐照单位进行复壮。
将来自16名志愿献血者的AS-1红细胞单位分成两个相等体积，每个单位的一个分装单位用25 Gy进行辐照。10个单位在4℃储存的第5、6或7天进行辐照，6个单位在4℃储存的第1天进行辐照。所有单位在4℃储存的第42天使用丙酮酸 - 肌苷 - 磷酸 - 腺嘌呤添加剂在37℃复壮1小时。对单位进行ATP、2,3 - DPG以及上清液钠、钾和葡萄糖的检测。
辐照和未辐照单位的ATP和2,3 - DPG水平恢复情况相同。证实了先前报道的辐照诱导的红细胞钾 - 钠转运。复壮完成1小时后，上清液钾和钠水平未逆转。在第1天或第5、6或7天辐照的单位之间结果无显著差异。
使用丙酮酸 - 肌苷 - 磷酸 - 腺嘌呤复壮添加剂，在4℃储存42天的辐照AS-1单位中，红细胞ATP和2,3 - DPG水平得以恢复。
