Wang R R, Zhang X Y
USDA-ARS (United States Department of Agriculture-Agricultural Research Service), Forage and Range Research Laboratory, Utah State University, Logan 84322-6300, USA.
Chromosome Res. 1996 Dec;4(8):583-7. doi: 10.1007/BF02261721.
Fluorescence in situ hybridization (FISH) was used to determine the breakpoint of the translocation chromosome in two bread wheat (Triticum aestivum) germplasm lines with Thinopyrum intermedium chromatin carrying resistance to either wheat streak mosaic virus (WSMV) or barley yellow dwarf virus (BYDV). In addition, genome-specific random amplified polymorphic DNA (RAPD) markers were used to ascertain the genomic sources of the Th. intermedium chromosome carrying the WSMV or BYDV resistance. CI17766, a WSMV-resistant wheat germplasm line derived from induced homoeologous pairing by using the ph1b mutant, had a translocation chromosome composed of the complete 4AL and about 45% of proximal 4AS from wheat, and the entire 4ES of Th. intermedium. The BYDV-resistant translocation line, TC14, derived from tissue culture, had a very short distal segment of 7StL from Th. intermedium terminally attached to 56% of the proximal 7DL. These observations indicate that translocations in these wheat germplasm lines did not involve centromeric breaks and fusion but were a result of homoeologous chromosomes recombination.
荧光原位杂交（FISH）被用于确定两个携带中间偃麦草染色质、对小麦线条花叶病毒（WSMV）或大麦黄矮病毒（BYDV）具有抗性的普通小麦（Triticum aestivum）种质系中易位染色体的断点。此外，利用基因组特异性随机扩增多态性DNA（RAPD）标记来确定携带WSMV或BYDV抗性的中间偃麦草染色体的基因组来源。CI17766是一个通过使用ph1b突变体诱导同源配对获得的抗WSMV小麦种质系，其易位染色体由完整的4AL和来自小麦的约45%的近端4AS以及中间偃麦草的整个4ES组成。来自组织培养的抗BYDV易位系TC14，其有一段来自中间偃麦草的非常短的7StL远端片段末端连接到近端7DL的56%处。这些观察结果表明，这些小麦种质系中的易位不涉及着丝粒的断裂和融合，而是同源染色体重组的结果。
