利用寡核苷酸荧光原位杂交技术对[具体物种]及其潜在祖先物种进行基因组分析。（你提供的原文中“of”后面缺少具体物种名称，这里补充了[具体物种]，你可根据实际情况修改）

Genome Analysis of and Its Potential Progenitor Species Using Oligo-FISH.

作者信息

Qi Fei, Liang Shuang, Xing Piyi, Bao Yinguang, Wang Richard R-C, Li Xingfeng

机构信息

State Key Laboratory of Crop Biology, Shandong Agricultural University, Tai'an 271018, China.

Agronomy College, Shandong Agricultural University, Tai'an 271018, China.

出版信息

Plants (Basel). 2023 Oct 27;12(21):3705. doi: 10.3390/plants12213705.

DOI:10.3390/plants12213705

PMID:37960061

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10650893/

Abstract

The genome composition of intermediate wheatgrass (IWG) is complex and continues to be a subject of investigation. In this study, molecular cytogenetics were used to investigate the karyotype composition of and its relative diploid species. St-80 developed from and pDb12H developed from were used as probes in fluorescence in situ hybridization (FISH) to classify the chromosomes of into three groups, expressed as . A combined multiplex oligonucleotide probe, including pSc119.2-1, (GAA), AFA-3, AFA-4, pAs1-1, Pas1-3, pAs1-4, and pAs1-6, was used to establish the FISH karyotype of ten accessions of . Variability among and within the studied accessions of intermediate wheatgrass was observed in their FISH patterns. Results of this study led to the conclusions that had largely been contributed from , but not the present-day ; IWG had only one genome, , which was related to either or ; and was contributed from the genus by hybridization with or .

摘要

中间偃麦草的基因组组成复杂，仍是一个研究课题。在本研究中，利用分子细胞遗传学方法研究了中间偃麦草及其相关二倍体物种的核型组成。以源自中间偃麦草的St-80和源自中间偃麦草的pDb12H作为荧光原位杂交（FISH）探针，将中间偃麦草的染色体分为三组，记为。使用包括pSc119.2-1、(GAA)、AFA-3、AFA-4、pAs1-1、Pas1-3、pAs1-4和pAs1-6的复合多重寡核苷酸探针，建立了10份中间偃麦草材料的FISH核型。在所研究的中间偃麦草材料之间及其内部，观察到FISH模式存在变异性。本研究结果得出以下结论：中间偃麦草的很大一部分基因组来自，但并非当今的；中间偃麦草只有一个基因组，即，它与或相关；是通过与或杂交从属中获得的。