Uklistaia T A, Trubnikov G A, Sukharev A E
Ter Arkh. 1996;68(10):22-8.
The examination of 150 patients with pleural exudate of different origin (43 cases of malignant and 107 cases of benign genesis) was made to elucidate diagnostic value of immunochemical serum and pleural fluid estimation of four tumor-associated proteins: carcinoembryonic antigen (CEA), beta 2-microglobulin (beta 2-MG), placental alkaline phosphatase (PAP), ferritin; three acute-phase proteins: C-reactive protein (CRP), lactoferrin, fibrin degradation products (FDP); expression of epithelial membrane antigen (EMA) in e date cells using monoclonal antibodies ICO-25. Determination CEA, beta 2-MG in the serum and pleural fluid, antituberculous an bodies in biological fluids proved diagnostically valuable for verification of pleural exudate characteristics. The discriminant analysis provided formulas for this differential diagnosis. The method identification in pleural fluid of cells expressing EMA with the use of monoclonal antibodies ICO-25 was found to be 2 times more efficient in detection of cancer cells than the standard light microscopy.
对150例不同病因的胸腔积液患者（43例恶性病因和107例良性病因）进行检查，以阐明免疫化学血清检测及四种肿瘤相关蛋白（癌胚抗原（CEA）、β2-微球蛋白（β2-MG）、胎盘碱性磷酸酶（PAP）、铁蛋白）和三种急性期蛋白（C反应蛋白（CRP）、乳铁蛋白、纤维蛋白降解产物（FDP））在胸腔积液中的诊断价值，以及使用单克隆抗体ICO-25检测上皮膜抗原（EMA）在间皮细胞中的表达。血清和胸腔积液中CEA、β2-MG的测定以及生物体液中抗结核抗体的检测对验证胸腔积液特征具有诊断价值。判别分析提供了这种鉴别诊断的公式。发现使用单克隆抗体ICO-25在胸腔积液中鉴定表达EMA的细胞的方法在检测癌细胞方面比标准光学显微镜效率高2倍。
