Hirao J, Hibi S, Andoh T, Ichimura T
First Department of Pediatrics, Dokkyo University School of Medicine, Tochigi, Japan.
Int Arch Allergy Immunol. 1997 Feb;112(2):152-6. doi: 10.1159/000237447.
For analysis of the cytokine network in Kawasaki disease (KD), we measured over time the plasma levels of interferon (IFN)-gamma, interleukin (IL)-4 and IL-10 in patients with KD. Fifteen patients with KD were studied. Eight healthy children were selected as control subjects. Circulating IFN-gamma levels were measured by immunoradiometric assay, and IL-4 and IL-10 levels were measured by enzyme-linked immunosorbent assay. The results were as follows: (1) The plasma levels of IFN-gamma in KD patients in the acute phase were significantly higher than the levels of patients in the convalescent phase (p < 0.05) and those of the control children (p < 0.05). (2) The plasma levels of IL-4 in the KD patients in the acute phase were significantly higher than the levels of the patients in the convalescent phase (p = 0.001) and those of the control children (p = 0.001). (3) The plasma levels of IL-10 in the KD patients in the acute phase were significantly higher than the levels of the patients in the convalescent phase (p < 0.03) and those of the control children (p < 0.005). (4) The investigation of the relationship between the IL-4 and IFN-gamma levels during the acute phase of KD demonstrated a significant reciprocal relationship (p < 0.05). (5) There was no significant relationship between the IL-4 and IL-10 levels during the acute phase. However, plasma IL-10 levels were low in the patients with high levels of plasma IL-4, and the patients with high levels of IL-10 revealed low levels of plasma IL-4. The above results suggested that a variety of patterns of cytokine production was present in the acute phase of this disease, and that the key cytokine, which might regulate the cytokine network, was IL-4.
为分析川崎病（KD）中的细胞因子网络，我们对KD患者的血浆干扰素（IFN）-γ、白细胞介素（IL）-4和IL-10水平进行了长期测量。研究了15例KD患者。选取8名健康儿童作为对照。采用免疫放射分析测定循环IFN-γ水平，采用酶联免疫吸附测定法测定IL-4和IL-10水平。结果如下：（1）急性期KD患者的血浆IFN-γ水平显著高于恢复期患者（p<0.05）和对照儿童（p<0.05）。（2）急性期KD患者的血浆IL-4水平显著高于恢复期患者（p=0.001）和对照儿童（p=0.001）。（3）急性期KD患者的血浆IL-10水平显著高于恢复期患者（p<0.03）和对照儿童（p<0.005）。（4）对KD急性期IL-4和IFN-γ水平之间关系的研究显示存在显著的负相关关系（p<0.05）。（5）急性期IL-4和IL-10水平之间无显著关系。然而，血浆IL-4水平高的患者血浆IL-10水平低，而IL-10水平高的患者血浆IL-4水平低。上述结果表明，该疾病急性期存在多种细胞因子产生模式，且可能调节细胞因子网络的关键细胞因子是IL-4。
