Vinogradov E V, Pantophlet R, Haseley S R, Brade L, Holst O, Brade H
Division of Biochemical Microbiology, Center for Medicine and Biosciences, Research Center Borstel, Germany.
Eur J Biochem. 1997 Jan 15;243(1-2):167-73. doi: 10.1111/j.1432-1033.1997.0167a.x.
S-form lipopolysaccharide was isolated by phenol/water extraction from a strain of Acinetobacter calcoaceticus (DNA group 1 ). The structure of the O-antigenic polysaccharide was determined by compositional analysis and NMR spectroscopy of the de-O-acylated lipopolysaccharide. The isolated polysaccharide obtained after hydrolysis of lipopolysaccharide in 0.01 M trifluoroacetic acid has the following structure: [STRUCTURE IN TEXT] in which Pyr is pyruvate. The O-acetyl substitution of D-Gal was non-stoichiometric. The O-antigen was specifically recognised in western blots by polyclonal rabbit antisera.
