Okabe H, Kishimoto Y
J Biol Chem. 1977 Oct 25;252(20):7068-73.
Three double-labeled ceramides, [1-14C]lignoceroyl D-erythro-[1-3H]sphingosine, [1-14C]palmitoyl D-erythro-[1-3H]sphingosine and D-[1-14C]cerebronoyl D-erythro-[3-3H]-sphingosine, were prepared and injected separately into the brains of 18-day-old rats. The animals were killed after 2 h and various sphingolipids were isolated and purified. These lipids were further fractionated into subgroups depending on their fatty acid content (nonhydroxy or alpha-hydroxy, shorter chain or longer chain), 3H/14C ratios obtained in ceramides, cerebrosides, and sphingomyelins containing different fatty acids from the injected material were much higher than the initial ratio. This observation indicates that a replacement of the fatty acid occurred in the injected ceramide. Comparison of the 3H/14C ratios of the cerebrosides and sphingomyelin in a specific fatty acid group with the ratios of the corresponding ceramide indicated that most of these lipids were derived directly from the ceramide. The 3H/14C ratio of the hydroxycerebroside obtained from cerebronyl sphingosine-injected brain was nearly twice the ratio of the injected material. In addition to these lipids, considerable radioactivity was incorporated into an unknown nonpolar material in the brains which had been injected with [1-14C]lignoceroyl or [1-14C]palmitoyl [1-3H]sphingosine. This material was tentatively identified as ceramide fatty acid ester. The 3H/14C ratio of the ester was identical to that of the injected material.
