Wigger-Alberti W, Hinnen U, Elsner P
Department of Dermatology, University of Zurich, Switzerland.
Contact Dermatitis. 1997 Jan;36(1):14-20. doi: 10.1111/j.1600-0536.1997.tb00916.x.
Metalworking fluids (MWF) have been reported as being an important cause of irritant contact dermatitis in metal workers. Our purpose was to determine whether the irritancy of different MWF assessed by 2 different types of predictive human in vivo tests could be compared with epidemiological data. 3 water-based MWF were tested in the same panel of subjects. Reactions were assessed by a visual score (VS), evaporimetry to evaluate the transepidermal water loss (TEWL) and chromametry to quantify erythema. Test 1: MWF were applied with Finn Chambers on the volunteers' mid-back, removed after 1 day of exposure, and reapplied for a further 2 days. Test 2: Cumulative irritant contact dermatitis was induced using a repetitive irritation test for 2 weeks (omitting weekends) for 6 h per day. We observed an increase in VS, TEWL, and erythema for all MWF, with the same irritancy ranking in both test models. Differentiation of the substances was better in the D1/D3 test. The experimental results partially correlated with the epidemiological data. Considering the shorter application time and the better discrimination of irritancy, we prefer the D1/D3 model as a predictive test of MWF irritancy. Our results might aid development of a standardized test to reduce cumulative skin irritation in metal workers.
据报道，金属加工液（MWF）是金属工人刺激性接触性皮炎的一个重要原因。我们的目的是确定通过两种不同类型的预测性人体体内试验评估的不同MWF的刺激性是否可以与流行病学数据进行比较。在同一组受试者中对3种水基金属加工液进行了测试。通过视觉评分（VS）、蒸发测定法评估经表皮水分流失（TEWL）以及比色法量化红斑来评估反应。试验1：将MWF用芬兰小室贴敷在志愿者的背部中间，暴露1天后取下，然后再贴敷2天。试验2：使用重复性刺激试验诱导累积性刺激性接触性皮炎，持续2周（周末除外），每天6小时。我们观察到所有MWF的VS、TEWL和红斑均增加，在两个试验模型中的刺激性排名相同。在D1/D3试验中物质的区分度更好。实验结果与流行病学数据部分相关。考虑到较短的应用时间和更好的刺激性辨别能力，我们更倾向于将D1/D3模型作为MWF刺激性的预测性试验。我们的结果可能有助于开发一种标准化试验，以减少金属工人的累积性皮肤刺激。
